Isolation of the pullulanase gene from Clostridium thermosulfurogenes (DSM 3896) and its expression in Escherichia coli

Isolation of the pullulanase gene from Clostridium thermosulfurogenes (DSM 3896) and its expression in Escherichia coli

The pullulanase gene from Clostridium thermosulfurogenes (DSM 3896) was cloned and expressed in Escherichia coli with pUC18 as cloning vector. Two clones showed expression of amylolytic enzymes which were active at high temperatures. One of the recombinant plasmids (pCT3) containing a 5.3 kbp insert...

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Veröffentlicht in:Current microbiology 1991-02, Vol.22 (2), p.91-95
Hauptverfasser: BURCHHARDT, G, WIENECKE, A, BAHL, H
Format: Artikel
Sprache:eng
Schlagworte:
Bacteriology
Biological and medical sciences
Clostridium thermosulfurigenes
Fundamental and applied biological sciences. Psychology
genes
Genetics
Microbiology
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Zusammenfassung:The pullulanase gene from Clostridium thermosulfurogenes (DSM 3896) was cloned and expressed in Escherichia coli with pUC18 as cloning vector. Two clones showed expression of amylolytic enzymes which were active at high temperatures. One of the recombinant plasmids (pCT3) containing a 5.3 kbp insert coded for the pullulanase gene; the other (pCT4, 4.4 kbp insert) carried the same alpha -amylase gene as the previously described plasmid pCT2 (2.9 kpb insert).
ISSN:0343-8651
1432-0991
DOI:10.1007/BF02105382