The use of diphenylene iodonium, an inhibitor of NADPH oxidase, to investigate the antimicrobial action of human monocyte derived macrophages

Diphenylene iodonium is an inhibitor of the respiratory burst-generating NADPH oxidase of phagocytes. The effect of this compound on human monocyte-derived macrophages and its usefulness in exploring the antimicrobial mechanisms of phagocytes was examined. 1 μM diphenylene iodonium inhibited hydroge...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of immunological methods 1990-10, Vol.133 (2), p.175-179
Hauptverfasser:	Robertson, A.K., Cross, A.R., Jones, O.T.G., Andrew, P.W.
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetylglucosaminidase - metabolism Animals Antimicrobial activity Cell Degranulation Cell Separation Cells, Cultured Diphenylene iodonium Female Humans Hydrogen peroxide Hydrogen Peroxide - metabolism Interferon-gamma - pharmacology Macrophage Macrophages - drug effects Macrophages - metabolism Macrophages - microbiology Male Mice NADH, NADPH Oxidoreductases - antagonists & inhibitors NADPH Oxidases Onium Compounds - pharmacology Oxygen - metabolism Phagocytes - drug effects Phagocytes - metabolism Phagocytes - microbiology Recombinant Proteins Tetradecanoylphorbol Acetate - pharmacology Toxoplasma Toxoplasma - physiology Toxoplasma gondii
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Diphenylene iodonium is an inhibitor of the respiratory burst-generating NADPH oxidase of phagocytes. The effect of this compound on human monocyte-derived macrophages and its usefulness in exploring the antimicrobial mechanisms of phagocytes was examined. 1 μM diphenylene iodonium inhibited hydrogen peroxide production by human macrophages and the activity of these cells against Toxoplasma gondii. At this concentration macrophage degranulation was unaffected.
ISSN:	0022-1759 1872-7905
DOI:	10.1016/0022-1759(90)90357-2