Linear amplification DNA sequencing directly from single phage plaques and bacterial colonies

Quicker ways of sequencing short DNA would facilitate both mutational analyses and long range mapping with sequence tagged sites (STS). The method of sequencing directly from plaques and colonies reported here is a labor-saving modification of the linear amplification method using partially purified DNAs. Our method is amenable to automation and is more efficient than those requiring prior PCR amplification when only < 200 bp of sequence are needed.