A vector for expressing foreign genes in the brains and hearts of transgenic mice
An expression plasmid (MoPrP.Xho), for use in transgenic mice, was developed from the promoter, 5′ intronic, and 3′ untranslated sequences of the murine prion protein gene. Analyses of mice harboring the MoPrP.Xho construct with cDNA genes encoding the amyloid precursor protein (APP) and human prese...
Gespeichert in:
Veröffentlicht in: | Genetic analysis : biomolecular engineering 1996-12, Vol.13 (6), p.159-163 |
---|---|
Hauptverfasser: | , , , , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | An expression plasmid (MoPrP.Xho), for use in transgenic mice, was developed from the promoter, 5′ intronic, and 3′ untranslated sequences of the murine prion protein gene. Analyses of mice harboring the MoPrP.Xho construct with cDNA genes encoding the amyloid precursor protein (APP) and human presenilin 1 demonstrated that this vector provides relatively high levels of transgene-encoded polypeptides in brains and hearts of transgenic mice. The MoPrP.Xho vector should be very useful in strategies designed to overexpress a variety of wild-type and disease related mutant transgenes in the heart and brain. |
---|---|
ISSN: | 1050-3862 |
DOI: | 10.1016/S1050-3862(96)00167-2 |