Polyclonal antibody-based ELISA and HPLC methods for the determination of fumonisins in corn: a comparative study
The performance of an experimental polyclonal antibody (PAb)-based competitive direct enzyme-linked immunosorbent assay (CD-ELISA) developed for the analysis of fumonisins in corn was assessed by comparison with an established high-perfommance liquid chromatography (HPLC) method. The comparative stu...
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| Veröffentlicht in: | Journal of food protection 1996-08, Vol.59 (8), p.893-897 |
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| container_end_page | 897 |
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| container_issue | 8 |
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| container_title | Journal of food protection |
| container_volume | 59 |
| creator | Sydenham, E.W. (Medical Research Council, Tygerberg, South Africa.) Stockenstrom, S Thiel, P.G Rheeder, J.P Doko, M.B Bird, C Miller, B.M |
| description | The performance of an experimental polyclonal antibody (PAb)-based competitive direct enzyme-linked immunosorbent assay (CD-ELISA) developed for the analysis of fumonisins in corn was assessed by comparison with an established high-perfommance liquid chromatography (HPLC) method. The comparative study was conducted using a series of 20 corn samples naturally contaminated with combined fumonisin levels ranging from 0.05 to 5 micrograms/g (ppm). Linear regression analysis between the results generated by HPLC and CD-ELISA provided correlation coefficients (r) and regression slopes (b) of r |
| doi_str_mv | 10.4315/0362-028X-59.8.893 |
| format | Article |
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Linear regression analysis between the results generated by HPLC and CD-ELISA provided correlation coefficients (r) and regression slopes (b) of r</description><identifier>ISSN: 0362-028X</identifier><identifier>EISSN: 1944-9097</identifier><identifier>DOI: 10.4315/0362-028X-59.8.893</identifier><identifier>PMID: 31159117</identifier><identifier>CODEN: JFPRDR</identifier><language>eng</language><publisher>Des Moines, IA: International Association of Milk, Food and Environmental Sanitarians</publisher><subject>ANTICORPS ; ANTICUERPOS ; Biological and medical sciences ; Cereal and baking product industries ; ELISA ; ENSAYO ; Food industries ; Food toxicology ; Fundamental and applied biological sciences. 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(Medical Research Council, Tygerberg, South Africa.)</creatorcontrib><creatorcontrib>Stockenstrom, S</creatorcontrib><creatorcontrib>Thiel, P.G</creatorcontrib><creatorcontrib>Rheeder, J.P</creatorcontrib><creatorcontrib>Doko, M.B</creatorcontrib><creatorcontrib>Bird, C</creatorcontrib><creatorcontrib>Miller, B.M</creatorcontrib><title>Polyclonal antibody-based ELISA and HPLC methods for the determination of fumonisins in corn: a comparative study</title><title>Journal of food protection</title><addtitle>J Food Prot</addtitle><description>The performance of an experimental polyclonal antibody (PAb)-based competitive direct enzyme-linked immunosorbent assay (CD-ELISA) developed for the analysis of fumonisins in corn was assessed by comparison with an established high-perfommance liquid chromatography (HPLC) method. The comparative study was conducted using a series of 20 corn samples naturally contaminated with combined fumonisin levels ranging from 0.05 to 5 micrograms/g (ppm). Linear regression analysis between the results generated by HPLC and CD-ELISA provided correlation coefficients (r) and regression slopes (b) of r</description><subject>ANTICORPS</subject><subject>ANTICUERPOS</subject><subject>Biological and medical sciences</subject><subject>Cereal and baking product industries</subject><subject>ELISA</subject><subject>ENSAYO</subject><subject>Food industries</subject><subject>Food toxicology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>HPLC</subject><subject>MAIS</subject><subject>MAIZ</subject><subject>METHODE STATISTIQUE</subject><subject>METODOS ESTADISTICOS</subject><subject>MICOTOXINAS</subject><subject>MYCOTOXINE</subject><subject>TEST ELISA</subject><subject>TESTAGE</subject><subject>Zea mays</subject><issn>0362-028X</issn><issn>1944-9097</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><recordid>eNpN0F1rFDEUgOEgil2rf6AXkgvxbtZ8TCZzvCtLtYUFC7XgXcjkw0Zmkm0yU9h_b5bdll4dCM85kBehC0rWLafiG-Edawjr_zQC1v26B_4GrSi0bQME5Fu0egFn6EMp_wghDFj3Hp1xSgVQKlfo8TaNezOmqEes4xyGZPfNoIuz-Gp7c3dZHy2-vt1u8OTmh2QL9inj-cFh62aXpxD1HFLEyWO_TCmGEmLBIWKTcvyOdZ3TTueKnhwu82L3H9E7r8fiPp3mObr_cfV7c91sf_282VxuG9Oydm6kkdAbISl3XDAYvOgkSCo6T4QQrWWyb63kBqglQgrggxOacGm4591AgZ-jr8e7u5weF1dmNYVi3Djq6NJS1KFAyzpSITtCk1Mp2Xm1y2HSea8oUYfQ6tBRHToqAapXNXRd-ny6vgyTsy8rz2Ur-HICuhg9-qyjCeWVA2A9reziyLxOSv_NldzfQccZq1_6D9DCjR8</recordid><startdate>19960801</startdate><enddate>19960801</enddate><creator>Sydenham, E.W. 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(Medical Research Council, Tygerberg, South Africa.)</creatorcontrib><creatorcontrib>Stockenstrom, S</creatorcontrib><creatorcontrib>Thiel, P.G</creatorcontrib><creatorcontrib>Rheeder, J.P</creatorcontrib><creatorcontrib>Doko, M.B</creatorcontrib><creatorcontrib>Bird, C</creatorcontrib><creatorcontrib>Miller, B.M</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of food protection</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sydenham, E.W. (Medical Research Council, Tygerberg, South Africa.)</au><au>Stockenstrom, S</au><au>Thiel, P.G</au><au>Rheeder, J.P</au><au>Doko, M.B</au><au>Bird, C</au><au>Miller, B.M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Polyclonal antibody-based ELISA and HPLC methods for the determination of fumonisins in corn: a comparative study</atitle><jtitle>Journal of food protection</jtitle><addtitle>J Food Prot</addtitle><date>1996-08-01</date><risdate>1996</risdate><volume>59</volume><issue>8</issue><spage>893</spage><epage>897</epage><pages>893-897</pages><issn>0362-028X</issn><eissn>1944-9097</eissn><coden>JFPRDR</coden><abstract>The performance of an experimental polyclonal antibody (PAb)-based competitive direct enzyme-linked immunosorbent assay (CD-ELISA) developed for the analysis of fumonisins in corn was assessed by comparison with an established high-perfommance liquid chromatography (HPLC) method. 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| fulltext | fulltext |
| identifier | ISSN: 0362-028X |
| ispartof | Journal of food protection, 1996-08, Vol.59 (8), p.893-897 |
| issn | 0362-028X 1944-9097 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_15914260 |
| source | Alma/SFX Local Collection; EZB Electronic Journals Library |
| subjects | ANTICORPS ANTICUERPOS Biological and medical sciences Cereal and baking product industries ELISA ENSAYO Food industries Food toxicology Fundamental and applied biological sciences. Psychology HPLC MAIS MAIZ METHODE STATISTIQUE METODOS ESTADISTICOS MICOTOXINAS MYCOTOXINE TEST ELISA TESTAGE Zea mays |
| title | Polyclonal antibody-based ELISA and HPLC methods for the determination of fumonisins in corn: a comparative study |
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