Polyclonal antibody-based ELISA and HPLC methods for the determination of fumonisins in corn: a comparative study

Polyclonal antibody-based ELISA and HPLC methods for the determination of fumonisins in corn: a comparative study

The performance of an experimental polyclonal antibody (PAb)-based competitive direct enzyme-linked immunosorbent assay (CD-ELISA) developed for the analysis of fumonisins in corn was assessed by comparison with an established high-perfommance liquid chromatography (HPLC) method. The comparative stu...

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Veröffentlicht in:Journal of food protection 1996-08, Vol.59 (8), p.893-897
Hauptverfasser: Sydenham, E.W. (Medical Research Council, Tygerberg, South Africa.), Stockenstrom, S, Thiel, P.G, Rheeder, J.P, Doko, M.B, Bird, C, Miller, B.M
Format: Artikel
Sprache:eng
Schlagworte:
ANTICORPS
ANTICUERPOS
Biological and medical sciences
Cereal and baking product industries
ELISA
ENSAYO
Food industries
Food toxicology
Fundamental and applied biological sciences. Psychology
HPLC
MAIS
MAIZ
METHODE STATISTIQUE
METODOS ESTADISTICOS
MICOTOXINAS
MYCOTOXINE
TEST ELISA
TESTAGE
Zea mays
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Zusammenfassung:The performance of an experimental polyclonal antibody (PAb)-based competitive direct enzyme-linked immunosorbent assay (CD-ELISA) developed for the analysis of fumonisins in corn was assessed by comparison with an established high-perfommance liquid chromatography (HPLC) method. The comparative study was conducted using a series of 20 corn samples naturally contaminated with combined fumonisin levels ranging from 0.05 to 5 micrograms/g (ppm). Linear regression analysis between the results generated by HPLC and CD-ELISA provided correlation coefficients (r) and regression slopes (b) of r
ISSN:0362-028X
1944-9097
DOI:10.4315/0362-028X-59.8.893