Development regulation of glucosidase I, an enzyme involved in the processing of asparagine-linked glycoproteins in rat mammary gland

Glucosidase I involved in the processing of N-linked glycoproteins was purified to homogeneity from the lactating rat mammary gland. The findings suggest that the accumulation of glucosidase I is modulated as a function of gland ontogeny. The immunoprecipitation of soluble extracts from ( super(35)S)methionine-labeled tissue with anti-rat alpha -lactalbumin antibodies indicates that these hormones not only stimulate the synthesis of alpha -lactalbumin but also play an important role in its glycosylation.