Biochemical characterization of FMDV A sub(10) and A sub(22) subtypes by PAGE and IEF

Biochemical characterization of FMDV A sub(10) and A sub(22) subtypes by PAGE and IEF

Both polyacrylamide gel electrophoresis (PAGE) and iso-electric focusing (IEF) have been standardized using the sucrose density gradient purified 146S particles of FMD virus subtypes A sub(10) and A sub(22). Differences in the molecular weights of structural proteins (VP sub(1), VP sub(2) and VP sub...

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Veröffentlicht in:Comparative immunology, microbiology and infectious diseases microbiology and infectious diseases, 1997-01, Vol.20 (1), p.95-99
Hauptverfasser: Nandi, S, Suryanarayana, VVS, Banumathi, N, Sen, A K
Format: Artikel
Sprache:eng ; fre
Schlagworte:
foot-and-mouth disease virus
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Zusammenfassung:Both polyacrylamide gel electrophoresis (PAGE) and iso-electric focusing (IEF) have been standardized using the sucrose density gradient purified 146S particles of FMD virus subtypes A sub(10) and A sub(22). Differences in the molecular weights of structural proteins (VP sub(1), VP sub(2) and VP sub(3) of two subtypes (A sub(10) and A sub(22)) of FMDV have been revealed in PAGE but no appreciable differences in the P sub(1) of VP sub(1), VP sub(2) and VP sub(3) is found in IEF.
ISSN:0147-9571