Cytotoxicity of human eosinophil granule major basic protein to human nasal sinus mucosa in vitro
The toxic effects of the human eosinophil granule major basic protein (MBP), reduced and alkylated, were studied on human nasal mucosa in vitro. With a microscope coupled with a television monitor (magnification ×2500) and videotape recorder, we investigated the effects of MBP on the mucosa and the...
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| Veröffentlicht in: | Journal of allergy and clinical immunology 1990-07, Vol.86 (1), p.52-63 |
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| container_title | Journal of allergy and clinical immunology |
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| creator | Hisamatsu, Ken-ichi Ganbo, Tetsuya Nakazawa, Tsutomu Murakami, Yoshihiko Gleich, Gerald J. Makiyama, Kiyoshi Koyama, Hideaki |
| description | The toxic effects of the human eosinophil granule major basic protein (MBP), reduced and alkylated, were studied on human nasal mucosa in vitro. With a microscope coupled with a television monitor (magnification ×2500) and videotape recorder, we investigated the effects of MBP on the mucosa and the ciliary activity of single cells. In nasal mucosal specimens from normal individuals, MBP, 5 μmol/L and 10 μmol/L, significantly inhibited (
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| doi_str_mv | 10.1016/S0091-6749(05)80123-X |
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p<0.01) ciliary activity by 4 and 1 hours of exposure, respectively. At these same MBP concentrations, the mucosal surface profiles were altered by 4 hours of exposure, and ciliostasis was 75% to 100% complete by 9 and 6 hours, respectively. In a mucosal specimen from a patient with nasal allergy, 1 μmol/L of MBP significantly inhibited (
p<0.01) ciliary activity by 1 hour; alteration of the mucosal surface profile appeared by 3 hours of exposure, and ciliostasis was 75% to 100% by 13 hours. Similar alterations of the mucosal surface profile were observed with specimens from a second patient with allergies; in contrast, 1 μmol/L of MPB had no effect on specimens from a nonallergic patient. These results indicate that MBP damages human upper respiratory epithelium, causing ciliostasis and alteration of the epithelial surface at concentrations likely achieved in vivo. Furthermore, the mucosal specimens from two allergic patients were damaged by concentrations of MBP that had no effect on mucosal specimens from a normal individual.</description><identifier>ISSN: 0091-6749</identifier><identifier>EISSN: 1097-6825</identifier><identifier>DOI: 10.1016/S0091-6749(05)80123-X</identifier><identifier>PMID: 2370389</identifier><identifier>CODEN: JACIBY</identifier><language>eng</language><publisher>New York, NY: Mosby, Inc</publisher><subject>Biological and medical sciences ; Blood Proteins - isolation & purification ; Blood Proteins - toxicity ; Cilia - drug effects ; Cilia - pathology ; Culture Techniques ; Dose-Response Relationship, Drug ; Eosinophil Granule Proteins ; Eosinophils - metabolism ; Ethmoid Sinus - drug effects ; Ethmoid Sinus - pathology ; Humans ; Immunopathology ; Maxillary Sinus - drug effects ; Maxillary Sinus - pathology ; Medical sciences ; Nasal Mucosa - drug effects ; Nasal Mucosa - pathology ; Respiratory Hypersensitivity - pathology ; Ribonucleases</subject><ispartof>Journal of allergy and clinical immunology, 1990-07, Vol.86 (1), p.52-63</ispartof><rights>1990 Mosby-Year Book, Inc.</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c448x-98d36c527421b9ec64028e39346e39b0dd5d351a5a46b59bbab6b9f6420342c3</citedby><cites>FETCH-LOGICAL-c448x-98d36c527421b9ec64028e39346e39b0dd5d351a5a46b59bbab6b9f6420342c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S009167490580123X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4503229$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2370389$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hisamatsu, Ken-ichi</creatorcontrib><creatorcontrib>Ganbo, Tetsuya</creatorcontrib><creatorcontrib>Nakazawa, Tsutomu</creatorcontrib><creatorcontrib>Murakami, Yoshihiko</creatorcontrib><creatorcontrib>Gleich, Gerald J.</creatorcontrib><creatorcontrib>Makiyama, Kiyoshi</creatorcontrib><creatorcontrib>Koyama, Hideaki</creatorcontrib><title>Cytotoxicity of human eosinophil granule major basic protein to human nasal sinus mucosa in vitro</title><title>Journal of allergy and clinical immunology</title><addtitle>J Allergy Clin Immunol</addtitle><description>The toxic effects of the human eosinophil granule major basic protein (MBP), reduced and alkylated, were studied on human nasal mucosa in vitro. With a microscope coupled with a television monitor (magnification ×2500) and videotape recorder, we investigated the effects of MBP on the mucosa and the ciliary activity of single cells. In nasal mucosal specimens from normal individuals, MBP, 5 μmol/L and 10 μmol/L, significantly inhibited (
p<0.01) ciliary activity by 4 and 1 hours of exposure, respectively. At these same MBP concentrations, the mucosal surface profiles were altered by 4 hours of exposure, and ciliostasis was 75% to 100% complete by 9 and 6 hours, respectively. In a mucosal specimen from a patient with nasal allergy, 1 μmol/L of MBP significantly inhibited (
p<0.01) ciliary activity by 1 hour; alteration of the mucosal surface profile appeared by 3 hours of exposure, and ciliostasis was 75% to 100% by 13 hours. Similar alterations of the mucosal surface profile were observed with specimens from a second patient with allergies; in contrast, 1 μmol/L of MPB had no effect on specimens from a nonallergic patient. These results indicate that MBP damages human upper respiratory epithelium, causing ciliostasis and alteration of the epithelial surface at concentrations likely achieved in vivo. Furthermore, the mucosal specimens from two allergic patients were damaged by concentrations of MBP that had no effect on mucosal specimens from a normal individual.</description><subject>Biological and medical sciences</subject><subject>Blood Proteins - isolation & purification</subject><subject>Blood Proteins - toxicity</subject><subject>Cilia - drug effects</subject><subject>Cilia - pathology</subject><subject>Culture Techniques</subject><subject>Dose-Response Relationship, Drug</subject><subject>Eosinophil Granule Proteins</subject><subject>Eosinophils - metabolism</subject><subject>Ethmoid Sinus - drug effects</subject><subject>Ethmoid Sinus - pathology</subject><subject>Humans</subject><subject>Immunopathology</subject><subject>Maxillary Sinus - drug effects</subject><subject>Maxillary Sinus - pathology</subject><subject>Medical sciences</subject><subject>Nasal Mucosa - drug effects</subject><subject>Nasal Mucosa - pathology</subject><subject>Respiratory Hypersensitivity - pathology</subject><subject>Ribonucleases</subject><issn>0091-6749</issn><issn>1097-6825</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtKxDAYRoMoOl4eQchCRBfV3NusRAZvILjQhbuQpKlG2mZM2sF5ezNOma2bXPjOl_wcAE4xusIIi-tXhCQuRMnkBeKXFcKEFu87YIaRLAtREb4LZlvkABym9IXynVZyH-wTWqJ8mgE9Xw1hCD_e-mEFQwM_x0730IXk-7D49C38iLofWwc7_RUiNDp5CxcxDM73cAgT3-ukW5g7Y4LdaEPSMMdLP8RwDPYa3SZ3Mu1H4O3-7m3-WDy_PDzNb58Ly1j1U8iqpsJyUjKCjXRWMEQqRyVlIq8G1TWvKceaayYMl8ZoI4xsBCOIMmLpETjfPJtn-x5dGlTnk3Vtq3sXxqQwr0osUZVBvgFtDClF16hF9J2OK4WRWptVf2bVWptCXP2ZVe-5dzp9MJrO1dvWpDLnZ1Ouk9Vtk7VZn7YY44gSssZuNpjLLpbeRZWsd711tY_ODqoO_p9BfgHG_JY3</recordid><startdate>199007</startdate><enddate>199007</enddate><creator>Hisamatsu, Ken-ichi</creator><creator>Ganbo, Tetsuya</creator><creator>Nakazawa, Tsutomu</creator><creator>Murakami, Yoshihiko</creator><creator>Gleich, Gerald J.</creator><creator>Makiyama, Kiyoshi</creator><creator>Koyama, Hideaki</creator><general>Mosby, Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope></search><sort><creationdate>199007</creationdate><title>Cytotoxicity of human eosinophil granule major basic protein to human nasal sinus mucosa in vitro</title><author>Hisamatsu, Ken-ichi ; Ganbo, Tetsuya ; Nakazawa, Tsutomu ; Murakami, Yoshihiko ; Gleich, Gerald J. ; Makiyama, Kiyoshi ; Koyama, Hideaki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c448x-98d36c527421b9ec64028e39346e39b0dd5d351a5a46b59bbab6b9f6420342c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Biological and medical sciences</topic><topic>Blood Proteins - isolation & purification</topic><topic>Blood Proteins - toxicity</topic><topic>Cilia - drug effects</topic><topic>Cilia - pathology</topic><topic>Culture Techniques</topic><topic>Dose-Response Relationship, Drug</topic><topic>Eosinophil Granule Proteins</topic><topic>Eosinophils - metabolism</topic><topic>Ethmoid Sinus - drug effects</topic><topic>Ethmoid Sinus - pathology</topic><topic>Humans</topic><topic>Immunopathology</topic><topic>Maxillary Sinus - drug effects</topic><topic>Maxillary Sinus - pathology</topic><topic>Medical sciences</topic><topic>Nasal Mucosa - drug effects</topic><topic>Nasal Mucosa - pathology</topic><topic>Respiratory Hypersensitivity - pathology</topic><topic>Ribonucleases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hisamatsu, Ken-ichi</creatorcontrib><creatorcontrib>Ganbo, Tetsuya</creatorcontrib><creatorcontrib>Nakazawa, Tsutomu</creatorcontrib><creatorcontrib>Murakami, Yoshihiko</creatorcontrib><creatorcontrib>Gleich, Gerald J.</creatorcontrib><creatorcontrib>Makiyama, Kiyoshi</creatorcontrib><creatorcontrib>Koyama, Hideaki</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Journal of allergy and clinical immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hisamatsu, Ken-ichi</au><au>Ganbo, Tetsuya</au><au>Nakazawa, Tsutomu</au><au>Murakami, Yoshihiko</au><au>Gleich, Gerald J.</au><au>Makiyama, Kiyoshi</au><au>Koyama, Hideaki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cytotoxicity of human eosinophil granule major basic protein to human nasal sinus mucosa in vitro</atitle><jtitle>Journal of allergy and clinical immunology</jtitle><addtitle>J Allergy Clin Immunol</addtitle><date>1990-07</date><risdate>1990</risdate><volume>86</volume><issue>1</issue><spage>52</spage><epage>63</epage><pages>52-63</pages><issn>0091-6749</issn><eissn>1097-6825</eissn><coden>JACIBY</coden><abstract>The toxic effects of the human eosinophil granule major basic protein (MBP), reduced and alkylated, were studied on human nasal mucosa in vitro. With a microscope coupled with a television monitor (magnification ×2500) and videotape recorder, we investigated the effects of MBP on the mucosa and the ciliary activity of single cells. In nasal mucosal specimens from normal individuals, MBP, 5 μmol/L and 10 μmol/L, significantly inhibited (
p<0.01) ciliary activity by 4 and 1 hours of exposure, respectively. At these same MBP concentrations, the mucosal surface profiles were altered by 4 hours of exposure, and ciliostasis was 75% to 100% complete by 9 and 6 hours, respectively. In a mucosal specimen from a patient with nasal allergy, 1 μmol/L of MBP significantly inhibited (
p<0.01) ciliary activity by 1 hour; alteration of the mucosal surface profile appeared by 3 hours of exposure, and ciliostasis was 75% to 100% by 13 hours. Similar alterations of the mucosal surface profile were observed with specimens from a second patient with allergies; in contrast, 1 μmol/L of MPB had no effect on specimens from a nonallergic patient. These results indicate that MBP damages human upper respiratory epithelium, causing ciliostasis and alteration of the epithelial surface at concentrations likely achieved in vivo. Furthermore, the mucosal specimens from two allergic patients were damaged by concentrations of MBP that had no effect on mucosal specimens from a normal individual.</abstract><cop>New York, NY</cop><pub>Mosby, Inc</pub><pmid>2370389</pmid><doi>10.1016/S0091-6749(05)80123-X</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of allergy and clinical immunology, 1990-07, Vol.86 (1), p.52-63 |
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| source | MEDLINE; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Biological and medical sciences Blood Proteins - isolation & purification Blood Proteins - toxicity Cilia - drug effects Cilia - pathology Culture Techniques Dose-Response Relationship, Drug Eosinophil Granule Proteins Eosinophils - metabolism Ethmoid Sinus - drug effects Ethmoid Sinus - pathology Humans Immunopathology Maxillary Sinus - drug effects Maxillary Sinus - pathology Medical sciences Nasal Mucosa - drug effects Nasal Mucosa - pathology Respiratory Hypersensitivity - pathology Ribonucleases |
| title | Cytotoxicity of human eosinophil granule major basic protein to human nasal sinus mucosa in vitro |
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