Analysis of the Hemolymph Proteins from the Acorn Barnacle Megabalanus rosa by Two-dimensional Gel Electrophoresis

The hemolymph proteins of the acorn barnacle Megabalanus rosa were separated by two-dimensional gel electrophoresis. More than 50 proteins were observed in the region of acidic (pl:4-7) with masses of 18-120 kDa. The proteins separated on the gel were electroblotted onto a polyvinylidene difluoride...

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Veröffentlicht in:Fisheries science 1996, Vol.62 (3), p.435-438
Hauptverfasser: Muramoto, Koji, Kuriki, Tomoko, Nokihara, Kiyoshi, Yako, Hiroshi, Kamiya, Hisao
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container_title Fisheries science
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creator Muramoto, Koji
Kuriki, Tomoko
Nokihara, Kiyoshi
Yako, Hiroshi
Kamiya, Hisao
description The hemolymph proteins of the acorn barnacle Megabalanus rosa were separated by two-dimensional gel electrophoresis. More than 50 proteins were observed in the region of acidic (pl:4-7) with masses of 18-120 kDa. The proteins separated on the gel were electroblotted onto a polyvinylidene difluoride membrane and were sequenced. Several major proteins were further characterized by amino acid analysis and peptide mapping, and identified to be multiple lectins.
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subjects Marine
Megabalanus rosa
title Analysis of the Hemolymph Proteins from the Acorn Barnacle Megabalanus rosa by Two-dimensional Gel Electrophoresis
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