Analysis of the Hemolymph Proteins from the Acorn Barnacle Megabalanus rosa by Two-dimensional Gel Electrophoresis

Analysis of the Hemolymph Proteins from the Acorn Barnacle Megabalanus rosa by Two-dimensional Gel Electrophoresis

The hemolymph proteins of the acorn barnacle Megabalanus rosa were separated by two-dimensional gel electrophoresis. More than 50 proteins were observed in the region of acidic (pl:4-7) with masses of 18-120 kDa. The proteins separated on the gel were electroblotted onto a polyvinylidene difluoride...

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Veröffentlicht in:Fisheries science 1996, Vol.62 (3), p.435-438
Hauptverfasser: Muramoto, Koji, Kuriki, Tomoko, Nokihara, Kiyoshi, Yako, Hiroshi, Kamiya, Hisao
Format: Artikel
Sprache:eng
Schlagworte:
Marine
Megabalanus rosa
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Zusammenfassung:The hemolymph proteins of the acorn barnacle Megabalanus rosa were separated by two-dimensional gel electrophoresis. More than 50 proteins were observed in the region of acidic (pl:4-7) with masses of 18-120 kDa. The proteins separated on the gel were electroblotted onto a polyvinylidene difluoride membrane and were sequenced. Several major proteins were further characterized by amino acid analysis and peptide mapping, and identified to be multiple lectins.
ISSN:0919-9268
DOI:10.2331/fishsci.62.435