A PCR-based approach for isolating pathogen resistance genes from potato with potential for wide application in plants

Plant genes for pathogen resistance can be used to engineer disease resistant crops. Oligonucleotides were designed from sequence motifs conserved between resistance genes of tobacco and Arabidopsis thaliana and used as PCR primers in potato DNA. Amplification products were obtained that were homolo...

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Veröffentlicht in:	Nature genetics 1996-12, Vol.14 (4), p.421-429
Hauptverfasser:	Leister, D. (Norwich Research Park, Norwich, UK.), Ballvora, A, Salamini, F, Gebhardt, C
Format:	Artikel
Sprache:	eng
Schlagworte:	ADN Amino Acid Sequence AMINO ACID SEQUENCES Arabidopsis - genetics ARABIDOPSIS THALIANA Biological and medical sciences BIOLOGICAL DIFFERENCES CARTE GENETIQUE CHEMICAL COMPOSITION Chromosome Mapping CHROMOSOME MAPS Classical genetics, quantitative genetics, hybrids COMPOSICION QUIMICA COMPOSITION CHIMIQUE Conserved Sequence DIFERENCIAS BIOLOGICAS DIFFERENCE BIOLOGIQUE DISEASE RESISTANCE DNA ENFERMEDADES DE LAS PLANTAS Fundamental and applied biological sciences. Psychology genbank/u60069 genbank/u60070 genbank/u60071 genbank/u60072 genbank/u60073 genbank/u60074 genbank/u60075 genbank/u60076 genbank/u60077 genbank/u60078 genbank/u60079 genbank/u60080 genbank/u60081 genbank/u60082 genbank/u60083 genbank/u60084 GENE GENES Genes, Plant GENETIC MAPPING GENETIC MAPS GENETICA GENETICS Genetics of eukaryotes. Biological and molecular evolution GENETIQUE ISOLATION LINKAGE GROUPS LOCI LOCUS Lycopersicon esculentum MALADIE DES PLANTES MAPAS GENETICOS Molecular Sequence Data MULTIGENE FAMILIES Nicotiana NICOTIANA TABACUM NUCLEOTIDE SEQUENCE PCR PEPTIDE PEPTIDES PEPTIDOS PESTS OF PLANTS Phytophthora infestans PLAGAS DE PLANTAS PLANT DISEASES Plant Diseases - genetics Plants - genetics Plants - microbiology Plants - parasitology Plants, Toxic POLYMERASE CHAIN REACTION Polymerase Chain Reaction - methods Polymorphism, Restriction Fragment Length POLYPEPTIDES Pteridophyta, spermatophyta RAVAGEUR DES PLANTES RESISTANCE AUX MALADIES RESISTENCIA A LA ENFERMEDAD RFLP SECUENCIA NUCLEOTIDICA SEQUENCE NUCLEOTIDIQUE SOLANUM TUBEROSUM Solanum tuberosum - genetics SPECIES DIFFERENCES STRUCTURAL GENES Vegetals
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Beschreibung
Zusammenfassung:	Plant genes for pathogen resistance can be used to engineer disease resistant crops. Oligonucleotides were designed from sequence motifs conserved between resistance genes of tobacco and Arabidopsis thaliana and used as PCR primers in potato DNA. Amplification products were obtained that were homologous to known resistance genes and linked without recombination with the nematode resistance locus Gro1 and the Phytophthora infestans resistance locus R7 of potato. Map positions of PCR-derived potato gene fragments were also correlated with resistance loci of the related tomato and tobacco genomes. Our results indicate that plant resistance genes that are effective against nematodes, fungi, viruses and bacteria may be isolated based on common sequence motifs and PCR methodology.
ISSN:	1061-4036 1546-1718
DOI:	10.1038/ng1296-421