highly regioselective synthesis of mannobiose and mannotriose by reverse hydrolysis using specific 1,2-alpha-mannosidase from Aspergillus phoenicis

A specific 1,2-alpha-mannosidase was isolated from A. phoenicis and used in the equilibrium-controlled synthesis of alpha 1 leads 2-linked mannobiose and mannotriose. Yields of 22.33% disaccharide, 8.21% trisaccharide and 2.74% tetrasaccharide were obtained. Regioselectivity was absolute for the 1 l...

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Veröffentlicht in:	Biotechnology letters 1996-07, Vol.18 (7), p.851-856
Hauptverfasser:	Suwasono, S, Rastall, R.A
Format:	Artikel
Sprache:	eng
Schlagworte:	Bioconversions. Hemisynthesis Biological and medical sciences Biological Sciences Biotechnology Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies
Online-Zugang:	Volltext
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Zusammenfassung:	A specific 1,2-alpha-mannosidase was isolated from A. phoenicis and used in the equilibrium-controlled synthesis of alpha 1 leads 2-linked mannobiose and mannotriose. Yields of 22.33% disaccharide, 8.21% trisaccharide and 2.74% tetrasaccharide were obtained. Regioselectivity was absolute for the 1 leads 2 linkage.
ISSN:	0141-5492 1573-6776
DOI:	10.1007/BF00127901