Adsorption of two cellobiohydrolases from Trichoderma reesei to Avicel: Evidence for "exo-exo" synergism and possible "loose complex" formation

Adsorption of two cellobiohydrolases from Trichoderma reesei to Avicel: Evidence for "exo-exo" synergism and possible "loose complex" formation

Pure cellobiohydrolases I and II (CBH I and II) from Trichoderma reesei adsorb strongly (K = 10 super(4) M super(-1)) onto micro-crystalline Avicel. Equilibration is slow (> 40 min) and saturation levels determined from the adsorption isotherms are almost identical: 107-110 nmoles enzyme/mg Avice...

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Veröffentlicht in:Biotechnology letters 1990-01, Vol.12 (7), p.525-530
Hauptverfasser: Tomme, P, Heriban, V, Claeyssens, M
Format: Artikel
Sprache:eng
Schlagworte:
cellobiohydrolase I
cellobiohydrolase II
immobilized enzymes
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Zusammenfassung:Pure cellobiohydrolases I and II (CBH I and II) from Trichoderma reesei adsorb strongly (K = 10 super(4) M super(-1)) onto micro-crystalline Avicel. Equilibration is slow (> 40 min) and saturation levels determined from the adsorption isotherms are almost identical: 107-110 nmoles enzyme/mg Avicel. In admixture synergistic effects are observed dependent on the ratio of the enzymes. These effects are maximal for non-saturating conditions (1-10 mu M) and when the enzymes are added in two consecutive steps synergism of binding is only apparent for CBH I.
ISSN:0141-5492
DOI:10.1007/BF01086347