Molecular cloning and expression of the rat beta sub(1)-adrenergic receptor gene

Using the sequence homology approach for cloning related genes within the G-protein-coupled receptor gene family, we have cloned the gene for the rat beta sub(1)-adrenergic receptor ( beta sub(1)-AR). The rat beta sub(1)-adrenergic receptor gene was isolated from a lambda EMBL3 rat genomic DNA library using the hamster beta sub(2)-adrenergic receptor ( beta sub(2)-AR) coding sequence as a probe under low stringency hybridization conditions. The rat beta sub(1)-AR gene encodes a protein of 466 amino acids that contains one consensus site for N-linked glycosylation (Asn-15) and three consensus sites for cAMP-dependent protein kinase phosphorylation (Ser-296, Ser-301, and Ser-401). The encoded rat beta sub(1)-AR is 98 and 91% similar at the amino acid level with the human beta sub(1)-AR in the transmembrane domains and in the overall sequence, respectively.