DNA-ligand interaction detected by proton selective and non-selective spin-lattice relaxation rate analysis

In this paper, a method based on NMR measurements for studying the ability of ligands to interact with macromolecule surfaces is proposed. It is based on temperature-dependent NMR proton selective and non-selective spin-lattice relaxation rate experiments and assumes fast chemical exchange conditions of the ligand between free and bound states. The results obtained appear able to identify DNA-psoralen complex formation. The use of this methodology for monitoring non-covalent interaction of ligands and bipolymers is suggested.