Formation and reversion of protoplasts in mutant strains of Brevibacterium sp. M27

In mutant strains of Brevibacterium sp. M27 requiring amino acids and resistant to antibiotics four methods were verified and a method inducing a high formation of protoplasts and their reversion was developed. Formation of protoplasts is 60-99% and their reversion 17-76%. In induced mutants a corre...

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Veröffentlicht in:Folia microbiologica 1990-01, Vol.35 (2), p.163-167
Hauptverfasser: Vecerkova, B, Konicek, J, Konickova-Radochova, M, Rytir, V
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Sprache:eng
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Zusammenfassung:In mutant strains of Brevibacterium sp. M27 requiring amino acids and resistant to antibiotics four methods were verified and a method inducing a high formation of protoplasts and their reversion was developed. Formation of protoplasts is 60-99% and their reversion 17-76%. In induced mutants a correlation between protoplast formation and growth rate could be demonstrated.
ISSN:0015-5632
DOI:10.1007/BF02820774