Three-dimensional microscopic computed tomography based on generalized Radon transform for optical imaging systems

We propose a 3D computed tomography method based on the generalized Radon transform defined for incoherent optical systems. We apply this method to reconstruct microscopic 3D images efficiently from 2D images acquired with multi-axis fluorescence microscopes which eliminate missing frequency bands....

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Veröffentlicht in:Optics communications 1996-02, Vol.123 (4), p.725-733
Hauptverfasser: Kikuchi, Susumu, Sonobe, Kazuo, Ohyama, Nagaaki
Format: Artikel
Sprache:eng
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Zusammenfassung:We propose a 3D computed tomography method based on the generalized Radon transform defined for incoherent optical systems. We apply this method to reconstruct microscopic 3D images efficiently from 2D images acquired with multi-axis fluorescence microscopes which eliminate missing frequency bands. Simulation results based on digital algorithms are shown.
ISSN:0030-4018
1873-0310
DOI:10.1016/0030-4018(95)00478-5