Binding and internalization of ricin labelled with fluorescein isothiocyanate

Binding and internalization of ricin labelled with fluorescein isothiocyanate

The toxic lectin ricin has been covalently labelled with fluorescein isothiocyanate on the enzymatically active A chain. The fluorescein reacted toxin maintains its biological activity. The lateral diffusion coefficient of cell surface bound ricin, studied in two cell lines by fluorescence photoblea...

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Veröffentlicht in:Biochemical and biophysical research communications 1990-06, Vol.169 (2), p.602-609
Hauptverfasser: Bellelli, A., Ippoliti, R., Brunori, M., Kam, Z., Benveniste, M., Emmanuel, F., Turpin, E., Alfsen, A., Frénoy, J.P.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Biological and medical sciences
Biological Transport
Cell physiology
Cell Survival - drug effects
Electrophoresis, Polyacrylamide Gel
Endocytosis
fluorescein isothiocyanate
Fluorescein-5-isothiocyanate
Fluoresceins
Fluorescent Dyes
Fundamental and applied biological sciences. Psychology
GRAINE
Hemagglutination Tests
Humans
Kinetics
LECTINA
LECTINE
LECTINS
Liver Neoplasms, Experimental
Macromolecular Substances
Molecular and cellular biology
Protein Binding
Rats
Ricin - isolation & purification
Ricin - metabolism
Ricin - pharmacology
RICINUS COMMUNIS
SEEDS
SEMILLA
THIOCYANATE
THIOCYANATES
TIOCIANATOS
Tumor Cells, Cultured - cytology
Tumor Cells, Cultured - drug effects
Tumor Cells, Cultured - metabolism
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Beschreibung
Zusammenfassung:The toxic lectin ricin has been covalently labelled with fluorescein isothiocyanate on the enzymatically active A chain. The fluorescein reacted toxin maintains its biological activity. The lateral diffusion coefficient of cell surface bound ricin, studied in two cell lines by fluorescence photobleaching recovery, is D = 1 − 2 × 10 −10 cm 2/s. Fluorescence microscopy provides preliminary evidence for secondary endosomes in the cytoplasm.
ISSN:0006-291X
1090-2104
DOI:10.1016/0006-291X(90)90373-U