Evidence for a role for L-proline as a salinity protectant in the cyanobacterium Nostoc muscorum

Nostoc muscorum required an active proline oxidase in order to assimilate exogenous proline as a source of fixed nitrogen. A mutant strain (Ac(r)) resistant to growth inhibition by L-azetidine-2-carboxylate (AC) was found to be deficient in proline oxidase activity, and to be a proline overaccumulator. Proline overaccumulation, resulting either from mutational acquisition of the Ac(r) phenotype or from salinity-inducible uptake of exogenous proline, conferred enhanced salinity tolerance in this cyanobacterium.