Supravital staining of eosinophils
Live eosinophils when mixed with Acridine Orange solution and viewed microscopically using u.v. light show very intense colours of their granules (yellow, orange and red) and green nuclear staining. Their active movement, translocation of granules and degranulation can be observed in vitro. Using th...
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| Veröffentlicht in: | International journal for parasitology 1996-04, Vol.26 (4), p.445-446 |
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| container_end_page | 446 |
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| container_issue | 4 |
| container_start_page | 445 |
| container_title | International journal for parasitology |
| container_volume | 26 |
| creator | Stankiewicz, M. Jonas, W. Hadas, E. Cabaj, W. Douch, P.G.C. |
| description | Live eosinophils when mixed with Acridine Orange solution and viewed microscopically using u.v. light show very intense colours of their granules (yellow, orange and red) and green nuclear staining. Their active movement, translocation of granules and degranulation can be observed
in vitro. Using this method, live eosinophils can be easily differentiated and enumerated. |
| doi_str_mv | 10.1016/0020-7519(96)00003-3 |
| format | Article |
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in vitro. Using this method, live eosinophils can be easily differentiated and enumerated.</description><identifier>ISSN: 0020-7519</identifier><identifier>EISSN: 1879-0135</identifier><identifier>DOI: 10.1016/0020-7519(96)00003-3</identifier><identifier>PMID: 8773533</identifier><identifier>CODEN: IJPYBT</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Acridine Orange ; Analysis of the immune response. Humoral and cellular immunity ; Animals ; Biological and medical sciences ; Cell Nucleus - ultrastructure ; Cytoplasmic Granules - physiology ; Cytoplasmic Granules - ultrastructure ; Eosinophils ; Eosinophils - chemistry ; Eosinophils - cytology ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; immunity ; Immunobiology ; Intestinal Mucosa - cytology ; Intestine, Small ; Leukocyte Count ; leukocytes ; Organs and cells involved in the immune response ; Sheep ; Staining and Labeling - methods ; supravital staining</subject><ispartof>International journal for parasitology, 1996-04, Vol.26 (4), p.445-446</ispartof><rights>1996</rights><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-cf74419fe08883e1bac747c4d94954075185aced06a19950ac1e8436e0ec8fe33</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0020751996000033$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3091129$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8773533$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Stankiewicz, M.</creatorcontrib><creatorcontrib>Jonas, W.</creatorcontrib><creatorcontrib>Hadas, E.</creatorcontrib><creatorcontrib>Cabaj, W.</creatorcontrib><creatorcontrib>Douch, P.G.C.</creatorcontrib><title>Supravital staining of eosinophils</title><title>International journal for parasitology</title><addtitle>Int J Parasitol</addtitle><description>Live eosinophils when mixed with Acridine Orange solution and viewed microscopically using u.v. light show very intense colours of their granules (yellow, orange and red) and green nuclear staining. Their active movement, translocation of granules and degranulation can be observed
in vitro. Using this method, live eosinophils can be easily differentiated and enumerated.</description><subject>Acridine Orange</subject><subject>Analysis of the immune response. Humoral and cellular immunity</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Nucleus - ultrastructure</subject><subject>Cytoplasmic Granules - physiology</subject><subject>Cytoplasmic Granules - ultrastructure</subject><subject>Eosinophils</subject><subject>Eosinophils - chemistry</subject><subject>Eosinophils - cytology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>immunity</subject><subject>Immunobiology</subject><subject>Intestinal Mucosa - cytology</subject><subject>Intestine, Small</subject><subject>Leukocyte Count</subject><subject>leukocytes</subject><subject>Organs and cells involved in the immune response</subject><subject>Sheep</subject><subject>Staining and Labeling - methods</subject><subject>supravital staining</subject><issn>0020-7519</issn><issn>1879-0135</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE9LxDAQxYMo67r6DRQWEdFDdWaTNslFkMV_sOBBPYdsOtVIt12TdsFvb8uWPZrLEOY3b-Y9xk4RbhAwuwWYQSJT1Fc6u4bu8YTvsTEqqRNAnu6z8Q45ZEcxfgNgyoUYsZGSkqecj9n5W7sOduMbW05jY33lq89pXUypjr6q11--jMfsoLBlpJOhTtjH48P7_DlZvD69zO8XiRMom8QVUgjUBYFSihMurZNCOpFroVMB3RUqtY5yyCxqnYJ1SErwjICcKojzCbvc6q5D_dNSbMzKR0dlaSuq22gwzSRHpTtQbEEX6hgDFWYd_MqGX4Ng-mhM79v0vo3uP100ptc_G_Tb5Yry3dCQRde_GPo2OlsWwVbOxx3GQSPO-u13W4y6LDaegonOU9U584FcY_La_3_HH3czfYE</recordid><startdate>19960401</startdate><enddate>19960401</enddate><creator>Stankiewicz, M.</creator><creator>Jonas, W.</creator><creator>Hadas, E.</creator><creator>Cabaj, W.</creator><creator>Douch, P.G.C.</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope></search><sort><creationdate>19960401</creationdate><title>Supravital staining of eosinophils</title><author>Stankiewicz, M. ; Jonas, W. ; Hadas, E. ; Cabaj, W. ; Douch, P.G.C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-cf74419fe08883e1bac747c4d94954075185aced06a19950ac1e8436e0ec8fe33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Acridine Orange</topic><topic>Analysis of the immune response. Humoral and cellular immunity</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell Nucleus - ultrastructure</topic><topic>Cytoplasmic Granules - physiology</topic><topic>Cytoplasmic Granules - ultrastructure</topic><topic>Eosinophils</topic><topic>Eosinophils - chemistry</topic><topic>Eosinophils - cytology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>immunity</topic><topic>Immunobiology</topic><topic>Intestinal Mucosa - cytology</topic><topic>Intestine, Small</topic><topic>Leukocyte Count</topic><topic>leukocytes</topic><topic>Organs and cells involved in the immune response</topic><topic>Sheep</topic><topic>Staining and Labeling - methods</topic><topic>supravital staining</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Stankiewicz, M.</creatorcontrib><creatorcontrib>Jonas, W.</creatorcontrib><creatorcontrib>Hadas, E.</creatorcontrib><creatorcontrib>Cabaj, W.</creatorcontrib><creatorcontrib>Douch, P.G.C.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>International journal for parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Stankiewicz, M.</au><au>Jonas, W.</au><au>Hadas, E.</au><au>Cabaj, W.</au><au>Douch, P.G.C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Supravital staining of eosinophils</atitle><jtitle>International journal for parasitology</jtitle><addtitle>Int J Parasitol</addtitle><date>1996-04-01</date><risdate>1996</risdate><volume>26</volume><issue>4</issue><spage>445</spage><epage>446</epage><pages>445-446</pages><issn>0020-7519</issn><eissn>1879-0135</eissn><coden>IJPYBT</coden><abstract>Live eosinophils when mixed with Acridine Orange solution and viewed microscopically using u.v. light show very intense colours of their granules (yellow, orange and red) and green nuclear staining. Their active movement, translocation of granules and degranulation can be observed
in vitro. Using this method, live eosinophils can be easily differentiated and enumerated.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>8773533</pmid><doi>10.1016/0020-7519(96)00003-3</doi><tpages>2</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | International journal for parasitology, 1996-04, Vol.26 (4), p.445-446 |
| issn | 0020-7519 1879-0135 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Acridine Orange Analysis of the immune response. Humoral and cellular immunity Animals Biological and medical sciences Cell Nucleus - ultrastructure Cytoplasmic Granules - physiology Cytoplasmic Granules - ultrastructure Eosinophils Eosinophils - chemistry Eosinophils - cytology Fundamental and applied biological sciences. Psychology Fundamental immunology immunity Immunobiology Intestinal Mucosa - cytology Intestine, Small Leukocyte Count leukocytes Organs and cells involved in the immune response Sheep Staining and Labeling - methods supravital staining |
| title | Supravital staining of eosinophils |
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