Supravital staining of eosinophils

Live eosinophils when mixed with Acridine Orange solution and viewed microscopically using u.v. light show very intense colours of their granules (yellow, orange and red) and green nuclear staining. Their active movement, translocation of granules and degranulation can be observed in vitro. Using th...

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Veröffentlicht in:	International journal for parasitology 1996-04, Vol.26 (4), p.445-446
Hauptverfasser:	Stankiewicz, M., Jonas, W., Hadas, E., Cabaj, W., Douch, P.G.C.
Format:	Artikel
Sprache:	eng
Schlagworte:	Acridine Orange Analysis of the immune response. Humoral and cellular immunity Animals Biological and medical sciences Cell Nucleus - ultrastructure Cytoplasmic Granules - physiology Cytoplasmic Granules - ultrastructure Eosinophils Eosinophils - chemistry Eosinophils - cytology Fundamental and applied biological sciences. Psychology Fundamental immunology immunity Immunobiology Intestinal Mucosa - cytology Intestine, Small Leukocyte Count leukocytes Organs and cells involved in the immune response Sheep Staining and Labeling - methods supravital staining
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Beschreibung
Zusammenfassung:	Live eosinophils when mixed with Acridine Orange solution and viewed microscopically using u.v. light show very intense colours of their granules (yellow, orange and red) and green nuclear staining. Their active movement, translocation of granules and degranulation can be observed in vitro. Using this method, live eosinophils can be easily differentiated and enumerated.
ISSN:	0020-7519 1879-0135
DOI:	10.1016/0020-7519(96)00003-3