Polarization and chemokinesis of ovine and human neutrophils in response to Fasciola hepatica excretory-secretory products
The effect of Fasciola hepatica excretory-secretory products (ES products) on the polarization and chemokinesis of sheep and human neutrophils was investigated. Flukes were cultured overnight in RPMI-1640 medium, and the resulting ES products concentrated by dialysis (cut-off 1.2 kDa) before use. At...
Gespeichert in:
| Veröffentlicht in: | International journal for parasitology 1996-04, Vol.26 (4), p.409-414 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 414 |
|---|---|
| container_issue | 4 |
| container_start_page | 409 |
| container_title | International journal for parasitology |
| container_volume | 26 |
| creator | Jefferies, J.R. Corbett, E. Barrett, J. Turner, R.J. |
| description | The effect of
Fasciola hepatica excretory-secretory products (ES products) on the polarization and chemokinesis of sheep and human neutrophils was investigated. Flukes were cultured overnight in RPMI-1640 medium, and the resulting ES products concentrated by dialysis (cut-off 1.2 kDa) before use. At the concentrations used toxicity tests showed no effect on neutrophil viability, but ES products were capable of causing morphological changes and chemokinesis in both sheep and human neutrophils. The polarizing effect of the ES products was found to be greater in the case of sheep neutrophils with 81% of cells polarizing at 80 μg total ES protein/ml as opposed to 36% of human neutrophils. Chemokinesis in response to 80 μg total ES protein/ml was sifnificant (
P |
| doi_str_mv | 10.1016/0020-7519(95)00136-0 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_15672958</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0020751995001360</els_id><sourcerecordid>15672958</sourcerecordid><originalsourceid>FETCH-LOGICAL-c417t-f883db9fc99687921321673a45b80d4de1e955a6e0821354ff9a883db0fbae153</originalsourceid><addsrcrecordid>eNp9kE9r3DAQxUVoSTdpvkEDOpSQHpxKtmVbl0BY8qcQSA_tWWjlEavGllyNHbL76avdNXvsSSPemzczP0K-cHbDGa--M5azrBZcXkvxjTFeVBk7IQve1DJLP_GBLI6WT-QM8U8yiaIsT8lpU9eFyJsF2f4MnY5uq0cXPNW-pWYNfXh1HtAhDZaGt1TvlfXUa089TGMMw9p1SJ2nEXAIHoGOgT5oNC7l0TUMKdBoCu8mwhjiJkOYKzrE0E5mxM_ko9UdwsX8npPfD_e_lk_Z88vjj-Xdc2ZKXo-ZbZqiXUlrpKzSaTkvcl7VhS7FqmFt2QIHKYSugDVJE6W1Uu9bmF1pSAefk6tDbhr8dwIcVe_QQNdpD2FCxUVV51I0yVgejCYGxAhWDdH1Om4UZ2qHXO14qh1PJYXaI1cstV3O-dOqh_bYNDNO-tdZT3h0Z6P2xuHRVjDJ5H7N24MNEos3B1ElmOANtC6CGVUb3P_3-AfOrZ87</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15672958</pqid></control><display><type>article</type><title>Polarization and chemokinesis of ovine and human neutrophils in response to Fasciola hepatica excretory-secretory products</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Jefferies, J.R. ; Corbett, E. ; Barrett, J. ; Turner, R.J.</creator><creatorcontrib>Jefferies, J.R. ; Corbett, E. ; Barrett, J. ; Turner, R.J.</creatorcontrib><description>The effect of
Fasciola hepatica excretory-secretory products (ES products) on the polarization and chemokinesis of sheep and human neutrophils was investigated. Flukes were cultured overnight in RPMI-1640 medium, and the resulting ES products concentrated by dialysis (cut-off 1.2 kDa) before use. At the concentrations used toxicity tests showed no effect on neutrophil viability, but ES products were capable of causing morphological changes and chemokinesis in both sheep and human neutrophils. The polarizing effect of the ES products was found to be greater in the case of sheep neutrophils with 81% of cells polarizing at 80 μg total ES protein/ml as opposed to 36% of human neutrophils. Chemokinesis in response to 80 μg total ES protein/ml was sifnificant (
P <0.01) in both species with sheep producing the stronger response. Delipidation of the ES products with Lipidex caused no loss of polarizing activity, nor did the purified lipid fraction show any polarizing effect. ES products lost polarizing activity after heat treatment or trypsin digestion. Preliminary fractionation on an S-300 Sephacryl column suggested the presence of at least four fractions capable of causing polarization in sheep neutrophils.</description><identifier>ISSN: 0020-7519</identifier><identifier>EISSN: 1879-0135</identifier><identifier>DOI: 10.1016/0020-7519(95)00136-0</identifier><identifier>PMID: 8773528</identifier><identifier>CODEN: IJPYBT</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Analysis of Variance ; Animals ; Biological and medical sciences ; chemokinesis ; Chemotaxis, Leukocyte ; excretory-secretory product ; Fasciola hepatica ; Fasciola hepatica - isolation & purification ; Fasciola hepatica - physiology ; Fascioliasis - parasitology ; Fascioliasis - veterinary ; Helminthic diseases ; Humans ; In Vitro Techniques ; Infectious diseases ; Liver - parasitology ; Medical sciences ; Microscopy, Electron, Scanning ; Miscellaneous ; neutrophils ; Neutrophils - diagnostic imaging ; Neutrophils - physiology ; Parasitic diseases ; polarization ; Sheep ; Sheep Diseases ; Trypsin ; Ultrasonography</subject><ispartof>International journal for parasitology, 1996-04, Vol.26 (4), p.409-414</ispartof><rights>1996</rights><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-f883db9fc99687921321673a45b80d4de1e955a6e0821354ff9a883db0fbae153</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0020-7519(95)00136-0$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3090915$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8773528$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jefferies, J.R.</creatorcontrib><creatorcontrib>Corbett, E.</creatorcontrib><creatorcontrib>Barrett, J.</creatorcontrib><creatorcontrib>Turner, R.J.</creatorcontrib><title>Polarization and chemokinesis of ovine and human neutrophils in response to Fasciola hepatica excretory-secretory products</title><title>International journal for parasitology</title><addtitle>Int J Parasitol</addtitle><description>The effect of
Fasciola hepatica excretory-secretory products (ES products) on the polarization and chemokinesis of sheep and human neutrophils was investigated. Flukes were cultured overnight in RPMI-1640 medium, and the resulting ES products concentrated by dialysis (cut-off 1.2 kDa) before use. At the concentrations used toxicity tests showed no effect on neutrophil viability, but ES products were capable of causing morphological changes and chemokinesis in both sheep and human neutrophils. The polarizing effect of the ES products was found to be greater in the case of sheep neutrophils with 81% of cells polarizing at 80 μg total ES protein/ml as opposed to 36% of human neutrophils. Chemokinesis in response to 80 μg total ES protein/ml was sifnificant (
P <0.01) in both species with sheep producing the stronger response. Delipidation of the ES products with Lipidex caused no loss of polarizing activity, nor did the purified lipid fraction show any polarizing effect. ES products lost polarizing activity after heat treatment or trypsin digestion. Preliminary fractionation on an S-300 Sephacryl column suggested the presence of at least four fractions capable of causing polarization in sheep neutrophils.</description><subject>Analysis of Variance</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>chemokinesis</subject><subject>Chemotaxis, Leukocyte</subject><subject>excretory-secretory product</subject><subject>Fasciola hepatica</subject><subject>Fasciola hepatica - isolation & purification</subject><subject>Fasciola hepatica - physiology</subject><subject>Fascioliasis - parasitology</subject><subject>Fascioliasis - veterinary</subject><subject>Helminthic diseases</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Infectious diseases</subject><subject>Liver - parasitology</subject><subject>Medical sciences</subject><subject>Microscopy, Electron, Scanning</subject><subject>Miscellaneous</subject><subject>neutrophils</subject><subject>Neutrophils - diagnostic imaging</subject><subject>Neutrophils - physiology</subject><subject>Parasitic diseases</subject><subject>polarization</subject><subject>Sheep</subject><subject>Sheep Diseases</subject><subject>Trypsin</subject><subject>Ultrasonography</subject><issn>0020-7519</issn><issn>1879-0135</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE9r3DAQxUVoSTdpvkEDOpSQHpxKtmVbl0BY8qcQSA_tWWjlEavGllyNHbL76avdNXvsSSPemzczP0K-cHbDGa--M5azrBZcXkvxjTFeVBk7IQve1DJLP_GBLI6WT-QM8U8yiaIsT8lpU9eFyJsF2f4MnY5uq0cXPNW-pWYNfXh1HtAhDZaGt1TvlfXUa089TGMMw9p1SJ2nEXAIHoGOgT5oNC7l0TUMKdBoCu8mwhjiJkOYKzrE0E5mxM_ko9UdwsX8npPfD_e_lk_Z88vjj-Xdc2ZKXo-ZbZqiXUlrpKzSaTkvcl7VhS7FqmFt2QIHKYSugDVJE6W1Uu9bmF1pSAefk6tDbhr8dwIcVe_QQNdpD2FCxUVV51I0yVgejCYGxAhWDdH1Om4UZ2qHXO14qh1PJYXaI1cstV3O-dOqh_bYNDNO-tdZT3h0Z6P2xuHRVjDJ5H7N24MNEos3B1ElmOANtC6CGVUb3P_3-AfOrZ87</recordid><startdate>19960401</startdate><enddate>19960401</enddate><creator>Jefferies, J.R.</creator><creator>Corbett, E.</creator><creator>Barrett, J.</creator><creator>Turner, R.J.</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope></search><sort><creationdate>19960401</creationdate><title>Polarization and chemokinesis of ovine and human neutrophils in response to Fasciola hepatica excretory-secretory products</title><author>Jefferies, J.R. ; Corbett, E. ; Barrett, J. ; Turner, R.J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-f883db9fc99687921321673a45b80d4de1e955a6e0821354ff9a883db0fbae153</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Analysis of Variance</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>chemokinesis</topic><topic>Chemotaxis, Leukocyte</topic><topic>excretory-secretory product</topic><topic>Fasciola hepatica</topic><topic>Fasciola hepatica - isolation & purification</topic><topic>Fasciola hepatica - physiology</topic><topic>Fascioliasis - parasitology</topic><topic>Fascioliasis - veterinary</topic><topic>Helminthic diseases</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Infectious diseases</topic><topic>Liver - parasitology</topic><topic>Medical sciences</topic><topic>Microscopy, Electron, Scanning</topic><topic>Miscellaneous</topic><topic>neutrophils</topic><topic>Neutrophils - diagnostic imaging</topic><topic>Neutrophils - physiology</topic><topic>Parasitic diseases</topic><topic>polarization</topic><topic>Sheep</topic><topic>Sheep Diseases</topic><topic>Trypsin</topic><topic>Ultrasonography</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jefferies, J.R.</creatorcontrib><creatorcontrib>Corbett, E.</creatorcontrib><creatorcontrib>Barrett, J.</creatorcontrib><creatorcontrib>Turner, R.J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>International journal for parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jefferies, J.R.</au><au>Corbett, E.</au><au>Barrett, J.</au><au>Turner, R.J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Polarization and chemokinesis of ovine and human neutrophils in response to Fasciola hepatica excretory-secretory products</atitle><jtitle>International journal for parasitology</jtitle><addtitle>Int J Parasitol</addtitle><date>1996-04-01</date><risdate>1996</risdate><volume>26</volume><issue>4</issue><spage>409</spage><epage>414</epage><pages>409-414</pages><issn>0020-7519</issn><eissn>1879-0135</eissn><coden>IJPYBT</coden><abstract>The effect of
Fasciola hepatica excretory-secretory products (ES products) on the polarization and chemokinesis of sheep and human neutrophils was investigated. Flukes were cultured overnight in RPMI-1640 medium, and the resulting ES products concentrated by dialysis (cut-off 1.2 kDa) before use. At the concentrations used toxicity tests showed no effect on neutrophil viability, but ES products were capable of causing morphological changes and chemokinesis in both sheep and human neutrophils. The polarizing effect of the ES products was found to be greater in the case of sheep neutrophils with 81% of cells polarizing at 80 μg total ES protein/ml as opposed to 36% of human neutrophils. Chemokinesis in response to 80 μg total ES protein/ml was sifnificant (
P <0.01) in both species with sheep producing the stronger response. Delipidation of the ES products with Lipidex caused no loss of polarizing activity, nor did the purified lipid fraction show any polarizing effect. ES products lost polarizing activity after heat treatment or trypsin digestion. Preliminary fractionation on an S-300 Sephacryl column suggested the presence of at least four fractions capable of causing polarization in sheep neutrophils.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>8773528</pmid><doi>10.1016/0020-7519(95)00136-0</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0020-7519 |
| ispartof | International journal for parasitology, 1996-04, Vol.26 (4), p.409-414 |
| issn | 0020-7519 1879-0135 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_15672958 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Analysis of Variance Animals Biological and medical sciences chemokinesis Chemotaxis, Leukocyte excretory-secretory product Fasciola hepatica Fasciola hepatica - isolation & purification Fasciola hepatica - physiology Fascioliasis - parasitology Fascioliasis - veterinary Helminthic diseases Humans In Vitro Techniques Infectious diseases Liver - parasitology Medical sciences Microscopy, Electron, Scanning Miscellaneous neutrophils Neutrophils - diagnostic imaging Neutrophils - physiology Parasitic diseases polarization Sheep Sheep Diseases Trypsin Ultrasonography |
| title | Polarization and chemokinesis of ovine and human neutrophils in response to Fasciola hepatica excretory-secretory products |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-20T13%3A34%3A53IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Polarization%20and%20chemokinesis%20of%20ovine%20and%20human%20neutrophils%20in%20response%20to%20Fasciola%20hepatica%20excretory-secretory%20products&rft.jtitle=International%20journal%20for%20parasitology&rft.au=Jefferies,%20J.R.&rft.date=1996-04-01&rft.volume=26&rft.issue=4&rft.spage=409&rft.epage=414&rft.pages=409-414&rft.issn=0020-7519&rft.eissn=1879-0135&rft.coden=IJPYBT&rft_id=info:doi/10.1016/0020-7519(95)00136-0&rft_dat=%3Cproquest_cross%3E15672958%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=15672958&rft_id=info:pmid/8773528&rft_els_id=0020751995001360&rfr_iscdi=true |