Polarization and chemokinesis of ovine and human neutrophils in response to Fasciola hepatica excretory-secretory products

The effect of Fasciola hepatica excretory-secretory products (ES products) on the polarization and chemokinesis of sheep and human neutrophils was investigated. Flukes were cultured overnight in RPMI-1640 medium, and the resulting ES products concentrated by dialysis (cut-off 1.2 kDa) before use. At the concentrations used toxicity tests showed no effect on neutrophil viability, but ES products were capable of causing morphological changes and chemokinesis in both sheep and human neutrophils. The polarizing effect of the ES products was found to be greater in the case of sheep neutrophils with 81% of cells polarizing at 80 μg total ES protein/ml as opposed to 36% of human neutrophils. Chemokinesis in response to 80 μg total ES protein/ml was sifnificant ( P