A Simple and Practicable PCR-Microchip with Bubble-Free Stationary Chamber

A Simple and Practicable PCR-Microchip with Bubble-Free Stationary Chamber

This paper reports on a simple and practicable biochip for polymerase chain reaction (PCR). The micro biochip (12mm×12mm) is a hybrid type which is composed of a polydimethylsiloxane (PDMS) cover with a hexagonal chamber and a glass substrate integrated with platinum (Pt) microheater and microsensor...

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Veröffentlicht in:Applied Mechanics and Materials 2013-12, Vol.483 (Mechanical Engineering, Materials and Energy III), p.51-55
Hauptverfasser: Cao, Jing Tong, Liu, Wu, Chen, Wen Yuan, Wu, Xiao Sheng, Zhang, Wei Ping, Cui, Feng, Luo, Jian
Format: Artikel
Sprache:eng
Schlagworte:
Biochips
Bubbles
Chambers
Chip formation
Chips
Platinum
Schedules
Silicone resins
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Beschreibung
Zusammenfassung:This paper reports on a simple and practicable biochip for polymerase chain reaction (PCR). The micro biochip (12mm×12mm) is a hybrid type which is composed of a polydimethylsiloxane (PDMS) cover with a hexagonal chamber and a glass substrate integrated with platinum (Pt) microheater and microsensor. Bubble formation has been reported as a big problem with PCR chips. In this paper we present a simple and practicable operation schedule to get a bubble free chip, in which a successful PCR process was guaranteed.
ISSN:1660-9336
1662-7482
1662-7482
DOI:10.4028/www.scientific.net/AMM.483.51