PRINS and in situ PCR protocols

Leading researchers demonstrate step-by-step how oligonucleotide primers may be successfully used to detect and amplify, or extend, complimentary sequences in situ. Through these procedures-often invented by the authors-the door is opened to rapid identification and characterization of chromosomal DNA sequences, viral genomes, and rare messenger RNAs in cells-at hitherto unmatched degrees of sensitivity and specificity. Their innovative techniques-suitable for both novice and experienced researchers-have rapidly become indispensable for many clinical diagnostic procedures, whether in the quantification of chromosomes for the identification of aneuploidy during prenatal diagnosis, the identification of early stages of viral infection by detection of rare viral genomes, or detection of low levels of gene expression and rare messenger RNAs.