Marinomonas profundimaris sp. nov., isolated from deep-sea sediment sample of the Arctic Ocean

A taxonomic study was carried out on strain D104ᵀ, which was isolated from deep-sea subsurface sediment sample from the Arctic Ocean. The bacterium was found to be Gram-negative, oxidase negative and catalase weakly positive, rod shaped, motile by means of polar flagellum. The organism grows between 4 and 37 °C (optimum 25–28 °C) and 0.5–6 % NaCl (optimum 3 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain D104ᵀ belongs to the genus Marinomonas, with highest sequence similarities of 97.7 % to Marinomonas ushuaiensis DSM 15871ᵀ, followed by M. dokdonensis DSW10-10ᵀ (96.9 %), M. arenicola KMM 3893ᵀ (96.7 %), M. arctica 328ᵀ (96.6 %) and other 18 species of the genus Marinomonas (94.4–96.5 %). The average nucleotide identity and estimated DNA–DNA hybridization values between strain D104ᵀ and M. ushuaiensis DSM 15871ᵀ were 84.24 % and 20.80 ± 2.33 % respectively. The principal fatty acids were C₁₆:₀, sum in feature 3 (C₁₆:₁ ω7c/C₁₆:₁ ω6c), sum in feature 8 (C₁₈:₁ ω7c/C₁₈:₁ ω6c) and C₁₂:₁ 3OH. The G + C content of the chromosomal DNA was determined to be 44.8 mol%. The respiratory quinone was found to be Q8 (100 %). Polar lipids include phosphatidylglycerol and phosphatidylethanolamine as major phospholipids and aminolipid and phospholipid as minor components. The results of the genotypic and phenotypic analyses indicate that strain D104ᵀ represents a novel species of the genus Marinomonas, for which the name Marinomonas profundimaris sp. nov. is proposed, with the type strain D104ᵀ (=MCCC 1A07573ᵀ = LMG 27696ᵀ).