Coriandrum sativum seed extract assisted in situ green synthesis of silver nanoparticle and its anti-microbial activity

We have developed a novel method to synthesize silver nanoparticles by mixing silver salt solution with seed extract of C. sativum without using any surfactant or external energy. By this method physiologically stable, bio-compatible Ag nanoparticles were formed which could be used for a variety of applications. With this method rapid synthesis of nanoparticles was observed to occur; i.e. reaction time was 1–2h as compared to 2–4 days required by microorganisms. TEM images shown reveals that there was poly-disperse spherical particles with nonuniform distribution of nanoparticles in the prepared sample. It was observed that the nanoparticles formed were of different sizes and particle size was found to be 9.94, 13.94, 15.91 and 12.59nm and the mean size of about 13.09nm which lies in the nano range. •Green synthesis of nanoparticles.•Antimicrobial activity of synthesized nanoparticles.•Synergetic effect study of nanoparticles and/with common antibiotic medicine penicillin. Extracellular in situ synthesis of metal nanoparticles using extracts of plants like Azadirachta indica (Neem), and Zingiber officinale (Ginger) has been successfully carried out earlier. In this study we have developed a novel method to synthesize silver nanoparticles by the process of bio-chemical reaction between silver salt solution with seed extract of Coriandrum sativum without using any surfactant or external energy. By this method physiologically stable, bio-compatible Ag nanoparticles (NPs) were formed. These functionalized AgNPs could be used for targeted drug delivery with enhanced therapeutic efficacy and minimal side effects. It is well known that on treating the metallic salt solution with some plant extracts, a rapid reduction occur leading to the formation of highly stable metal nanoparticles. This method resulted in rapid synthesis of nanoparticles, i.e. reaction time was 1–2h as compared to 2–4 days required by microorganisms. These nanoparticles were analysed by various characterization techniques to reveal their morphology and chemical composition. TEM image of these NPs indicated the formation of spherical, non-uniform, poly dispersed nanoparticles of mean size of 13.09nm. A detailed study of anti-microbial activity of nanoparticles and its synergetic effectiveness with common narrow spectrum antibiotic has been successfully carried out.