Immobilization of Catalase on Electrospun PVA/PA6–Cu(II) Nanofibrous Membrane for the Development of Efficient and Reusable Enzyme Membrane Reactor

In this study, a mat/membrane consisting of overlaid PVA/PA6–Cu­(II) composite nanofibers was prepared via the electrospinning technique followed by coordination/chelation with Cu­(II) ions; an enzyme of catalase (CAT) was then immobilized onto the PVA/PA6–Cu­(II) nanofibrous membrane. The amount of immobilized catalase reached a high value of 64 ± 4.6 mg/g, while the kinetic parameters (V max and K m) of enzyme were 3774 μmol/mg·min and 41.13 mM, respectively. Furthermore, the thermal stability and storage stability of immobilized catalase were improved significantly. Thereafter, a plug-flow type of immobilized enzyme membrane reactor (IEMR) was assembled from the PVA/PA6–Cu­(II)–CAT membrane. With the increase of operational pressure from 0.02 to 0.2 MPa, the flux value of IEMR increased from 0.20 ± 0.02 to 0.76 ± 0.04 L/m2·min, whereas the conversion ratio of H2O2 decreased slightly from 92 ± 2.5% to 87 ± 2.1%. After 5 repeating cycles, the production capacity of IEMR was merely decreased from 0.144 ± 0.006 to 0.102 ± 0.004 mol/m2·min. These results indicated that the assembled IEMR possessed high productivity and excellent reusability, suggesting that the IEMR based on electrospun PVA/PA6–Cu­(II) nanofibrous membrane might have great potential for various applications, particularly those related to environmental protection.