Reconstruction of a seminiferous tubule-like structure in a 3 dimensional culture system of re-aggregated mouse neonatal testicular cells within a collagen matrix

Highlights • Seminiferous tubule-like structures are reconstructed by 3-D re-aggregate culture. • KnockOut Serum Replacement promotes reconstruction of tubule-like structures. • Germ cells are sorted inside, and Leydig cells are outside tubule-like structures. • Polar Sertoli cells form tight junctions and lumen in tubule-like structures. • Spermatogonia proliferate and differentiate into primary spermatocytes.