Dysgonomonas macrotermitis sp. nov., isolated from the hindgut of a fungus-growing termite

A Gram-stain-negative, facultatively anaerobic, non-motile and coccoid- to short-rod-shaped bacterium, designated strain Dys-CH1T, was isolated from the hindgut of a fungus-growing termite Macrotermes barneyi. The optimal pH and cultivation temperature of strain Dys-CH1T were pH 7.2–7.6 and 35–37 °C, respectively. Sequence analysis of 16S rRNA gene showed that Dys-CH1T shared 94.6 % and 90.9 % similarity with Dysgonomonas capnocytophagoides JCM 16697T and Dysgonomonas gadei CCUG 42882T, respectively. Strain Dys-CH1T was found to be different from other species of the genus Dysgonomonas with validly published names with respect to taxonomically important traits, including habitat, biochemical tests, DNA G+C content, bile resistance, fatty-acid composition and susceptibility to antimicrobial agents. On the basis of these characteristics, strain Dys-CH1T represents a novel species of the genus Dysgonomonas for which the name Dysgonomonas macrotermitis sp. nov. is proposed. The type strain is Dys-CH1T ( = JCM 19375T = DSM 27370T).