Fluorescence properties of the Ca super(2+), Mg super(2+)-ATPase protein of sarcoplasmic reticulum labeled with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole

Fluorescence properties of the Ca super(2+), Mg super(2+)-ATPase protein of sarcoplasmic reticulum labeled with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole

The fluorescence intensity of the Ca super(2+), Mg super(2+)-ATPase protein of rabbit skeletal sarcoplasmic reticulum that incorporated about 2 mol of 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) was enhanced at high MgATP concentrations with or without 50 mu M calcium. The observed enhancement...

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Veröffentlicht in:FEBS letters 1990-01, Vol.259 (2), p.254-256
Hauptverfasser: Bailin, G, Huang, J R
Format: Artikel
Sprache:eng
Schlagworte:
Ca super(2+)/Mg super(2+)-transporting ATPase
sarcoplasmic reticulum
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Zusammenfassung:The fluorescence intensity of the Ca super(2+), Mg super(2+)-ATPase protein of rabbit skeletal sarcoplasmic reticulum that incorporated about 2 mol of 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) was enhanced at high MgATP concentrations with or without 50 mu M calcium. The observed enhancement indicates that the fluorophore, NBD-Cl, can detect conformational changes in the ATPase protein.
ISSN:0014-5793