Effects of four additive solutions on canine leukoreduced red cell concentrate quality during storage

Background Additive solutions (AS) and prestorage leukoreduction (LR) are important tools used to maintain erythrocyte viability during storage and avoid transfusion reactions in recipients, respectively. Objectives The purpose of the study was to determine the efficacy of a WBC filter (Immugard IIIRC) and compare the effect of 4 AS (phosphate‐adenine‐glucose‐guanosine‐gluconate‐mannitol [PAGGGM], saline‐adenine‐glucose‐mannitol [SAGM], Adsol, Optisol) on the in vitro quality of canine leukoreduced packed RBC units (pRBC) stored for 41 days. Methods Five hundred milliliters of blood were collected from 8 healthy dogs each into 70 mL of citrate‐phosphate‐dextrose (CPD) solution, and were leukoreduced by a polyurethane filter. pRBC of each dog were divided equally into 4 bags containing a different AS. Bags were stored for 41 days at 4°C and evaluated every 10 days. Variables analyzed included pH, PCV, and% hemolysis, and lactate, glucose, potassium, sodium, ATP, and 2,3‐diphosphoglycerate (2,3‐DPG) concentrations. Results The LR resulted in residual WBC counts comparable to human standards. During storage, pH, and glucose, 2,3‐DPG, and ATP concentrations decreased, and hemolysis, and lactate, sodium, and potassium concentrations increased (P