Capacity of multidrug-resistant clinical isolates of Acinetobacter baumannii to form biofilm and adhere to epithelial cell surfaces
This study evaluated the capacity of 23 multidrug-resistant (MDR) clinical isolates of Acinetobacter baumannii to adhere to respiratory epithelial cell surfaces and to form biofilm on a polystyrene surface. All 23 A. baumannii isolates were capable of adhering efficiently to respiratory epithelial c...
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| Veröffentlicht in: | Clinical microbiology and infection 2008-01, Vol.14 (1), p.49-54 |
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| creator | Lee, H.-W. Koh, Y.M. Kim, J. Lee, J.-C. Lee, Y.-C. Seol, S.-Y. Cho, D.-T. Kim, J. |
| description | This study evaluated the capacity of 23 multidrug-resistant (MDR) clinical isolates of Acinetobacter baumannii to adhere to respiratory epithelial cell surfaces and to form biofilm on a polystyrene surface. All 23 A. baumannii isolates were capable of adhering efficiently to respiratory epithelial cells, and biofilm production was positively associated with epithelial cell adhesiveness (r 0.80, p |
| doi_str_mv | 10.1111/j.1469-0691.2007.01842.x |
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All 23 A. baumannii isolates were capable of adhering efficiently to respiratory epithelial cells, and biofilm production was positively associated with epithelial cell adhesiveness (r 0.80, p <0.0001). In the presence of the chelating agent EDTA, biofilm formation was markedly reduced. Cell adhesiveness and biofilm formation were significantly higher in isolates carrying the blaPER-1 gene as compared with isolates without this extended-spectrum β-lactamase gene (p <0.005 and p <0.001, respectively). Further examination by RT-PCR showed a positive correlation between the level of expression of the blaPER-1 gene and the level of biofilm formation (r 0.89, p <0.0001) and cell adhesiveness (r 0.74, p <0.006). Overall, the study demonstrated a high capacity of clinical isolates of MDR A. baumannii to form biofilm and to adhere to respiratory epithelial cells. This feature, combined with multidrug resistance, might contribute to the survival of these organisms and their dissemination in the hospital environment.</description><identifier>ISSN: 1198-743X</identifier><identifier>EISSN: 1469-0691</identifier><identifier>DOI: 10.1111/j.1469-0691.2007.01842.x</identifier><identifier>PMID: 18005176</identifier><language>eng</language><publisher>Oxford, UK: Elsevier Ltd</publisher><subject>Acinetobacter baumannii ; Acinetobacter baumannii - drug effects ; Acinetobacter baumannii - genetics ; Acinetobacter baumannii - physiology ; Acinetobacter Infections - microbiology ; adherence ; Anti-Bacterial Agents - pharmacology ; Bacterial Adhesion - physiology ; Bacteriology ; beta-Lactamases - genetics ; biofilm formation on epithelial cells ; Biofilms - drug effects ; Biofilms - growth & development ; Biological and medical sciences ; Cell Line ; Drug Resistance, Multiple, Bacterial ; Edetic Acid - pharmacology ; Epithelial Cells - microbiology ; Fundamental and applied biological sciences. Psychology ; Humans ; Microbiology ; multidrug resistance ; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains ; PER-1 β-lactamase ; Respiratory Mucosa - microbiology</subject><ispartof>Clinical microbiology and infection, 2008-01, Vol.14 (1), p.49-54</ispartof><rights>2008 European Society of Clinical Infectious Diseases</rights><rights>2007 The Authors</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5742-fe3c9f087760f63e3159574796c80caca183719295cde76fc83f4db48aac8b323</citedby><cites>FETCH-LOGICAL-c5742-fe3c9f087760f63e3159574796c80caca183719295cde76fc83f4db48aac8b323</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1469-0691.2007.01842.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1469-0691.2007.01842.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,4010,27900,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19984166$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18005176$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, H.-W.</creatorcontrib><creatorcontrib>Koh, Y.M.</creatorcontrib><creatorcontrib>Kim, J.</creatorcontrib><creatorcontrib>Lee, J.-C.</creatorcontrib><creatorcontrib>Lee, Y.-C.</creatorcontrib><creatorcontrib>Seol, S.-Y.</creatorcontrib><creatorcontrib>Cho, D.-T.</creatorcontrib><creatorcontrib>Kim, J.</creatorcontrib><title>Capacity of multidrug-resistant clinical isolates of Acinetobacter baumannii to form biofilm and adhere to epithelial cell surfaces</title><title>Clinical microbiology and infection</title><addtitle>Clin Microbiol Infect</addtitle><description>This study evaluated the capacity of 23 multidrug-resistant (MDR) clinical isolates of Acinetobacter baumannii to adhere to respiratory epithelial cell surfaces and to form biofilm on a polystyrene surface. All 23 A. baumannii isolates were capable of adhering efficiently to respiratory epithelial cells, and biofilm production was positively associated with epithelial cell adhesiveness (r 0.80, p <0.0001). In the presence of the chelating agent EDTA, biofilm formation was markedly reduced. Cell adhesiveness and biofilm formation were significantly higher in isolates carrying the blaPER-1 gene as compared with isolates without this extended-spectrum β-lactamase gene (p <0.005 and p <0.001, respectively). Further examination by RT-PCR showed a positive correlation between the level of expression of the blaPER-1 gene and the level of biofilm formation (r 0.89, p <0.0001) and cell adhesiveness (r 0.74, p <0.006). Overall, the study demonstrated a high capacity of clinical isolates of MDR A. baumannii to form biofilm and to adhere to respiratory epithelial cells. This feature, combined with multidrug resistance, might contribute to the survival of these organisms and their dissemination in the hospital environment.</description><subject>Acinetobacter baumannii</subject><subject>Acinetobacter baumannii - drug effects</subject><subject>Acinetobacter baumannii - genetics</subject><subject>Acinetobacter baumannii - physiology</subject><subject>Acinetobacter Infections - microbiology</subject><subject>adherence</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Bacterial Adhesion - physiology</subject><subject>Bacteriology</subject><subject>beta-Lactamases - genetics</subject><subject>biofilm formation on epithelial cells</subject><subject>Biofilms - drug effects</subject><subject>Biofilms - growth & development</subject><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>Drug Resistance, Multiple, Bacterial</subject><subject>Edetic Acid - pharmacology</subject><subject>Epithelial Cells - microbiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Microbiology</subject><subject>multidrug resistance</subject><subject>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</subject><subject>PER-1 β-lactamase</subject><subject>Respiratory Mucosa - microbiology</subject><issn>1198-743X</issn><issn>1469-0691</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkT-P1DAQxSME4o6Dr4DcINEk2HE2tguKY8U_aRENSHSW44y5WTnxYjvHbc0Xx2FXXAluPNL7vfH4TVURRhtWzqt9w7pe1bRXrGkpFQ1lsmubuwfV5V_hYamZkrXo-LeL6klKe0ppy3n3uLpgktINE_1l9WtrDsZiPpLgyLT4jGNcvtcREqZs5kysxxmt8QRT8CZDWsFrizPkMBibIZLBLJOZZ0SSA3EhTmTA4NBPxMwjMeMNRFglOGC-AY-lmQXvSVqiMxbS0-qRMz7Bs_N9VX199_bL9kO9-_z-4_Z6V9uN6NraAbfKUSlET13PgbONKoJQvZXUGmuY5IKpVm3sCKJ3VnLXjUMnjbFy4C2_ql6e-h5i-LFAynrCtE5iZghL0mzTU8ZF6V9QeUJtDClFcPoQcTLxqBnV6wr0Xq9J6zVpva5A_1mBvivW5-dXlmGC8d54zrwAL86ASSVYF81sMd1zSsmO9Sv3-sT9RA_H_x5Ab3ef1qr435z8UDK9RYg6WYTZwogRbNZjwH__5jczG7k-</recordid><startdate>200801</startdate><enddate>200801</enddate><creator>Lee, H.-W.</creator><creator>Koh, Y.M.</creator><creator>Kim, J.</creator><creator>Lee, J.-C.</creator><creator>Lee, Y.-C.</creator><creator>Seol, S.-Y.</creator><creator>Cho, D.-T.</creator><creator>Kim, J.</creator><general>Elsevier Ltd</general><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>200801</creationdate><title>Capacity of multidrug-resistant clinical isolates of Acinetobacter baumannii to form biofilm and adhere to epithelial cell surfaces</title><author>Lee, H.-W. ; Koh, Y.M. ; Kim, J. ; Lee, J.-C. ; Lee, Y.-C. ; Seol, S.-Y. ; Cho, D.-T. ; Kim, J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5742-fe3c9f087760f63e3159574796c80caca183719295cde76fc83f4db48aac8b323</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Acinetobacter baumannii</topic><topic>Acinetobacter baumannii - drug effects</topic><topic>Acinetobacter baumannii - genetics</topic><topic>Acinetobacter baumannii - physiology</topic><topic>Acinetobacter Infections - microbiology</topic><topic>adherence</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Bacterial Adhesion - physiology</topic><topic>Bacteriology</topic><topic>beta-Lactamases - genetics</topic><topic>biofilm formation on epithelial cells</topic><topic>Biofilms - drug effects</topic><topic>Biofilms - growth & development</topic><topic>Biological and medical sciences</topic><topic>Cell Line</topic><topic>Drug Resistance, Multiple, Bacterial</topic><topic>Edetic Acid - pharmacology</topic><topic>Epithelial Cells - microbiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Microbiology</topic><topic>multidrug resistance</topic><topic>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</topic><topic>PER-1 β-lactamase</topic><topic>Respiratory Mucosa - microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, H.-W.</creatorcontrib><creatorcontrib>Koh, Y.M.</creatorcontrib><creatorcontrib>Kim, J.</creatorcontrib><creatorcontrib>Lee, J.-C.</creatorcontrib><creatorcontrib>Lee, Y.-C.</creatorcontrib><creatorcontrib>Seol, S.-Y.</creatorcontrib><creatorcontrib>Cho, D.-T.</creatorcontrib><creatorcontrib>Kim, J.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Clinical microbiology and infection</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, H.-W.</au><au>Koh, Y.M.</au><au>Kim, J.</au><au>Lee, J.-C.</au><au>Lee, Y.-C.</au><au>Seol, S.-Y.</au><au>Cho, D.-T.</au><au>Kim, J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Capacity of multidrug-resistant clinical isolates of Acinetobacter baumannii to form biofilm and adhere to epithelial cell surfaces</atitle><jtitle>Clinical microbiology and infection</jtitle><addtitle>Clin Microbiol Infect</addtitle><date>2008-01</date><risdate>2008</risdate><volume>14</volume><issue>1</issue><spage>49</spage><epage>54</epage><pages>49-54</pages><issn>1198-743X</issn><eissn>1469-0691</eissn><abstract>This study evaluated the capacity of 23 multidrug-resistant (MDR) clinical isolates of Acinetobacter baumannii to adhere to respiratory epithelial cell surfaces and to form biofilm on a polystyrene surface. All 23 A. baumannii isolates were capable of adhering efficiently to respiratory epithelial cells, and biofilm production was positively associated with epithelial cell adhesiveness (r 0.80, p <0.0001). In the presence of the chelating agent EDTA, biofilm formation was markedly reduced. Cell adhesiveness and biofilm formation were significantly higher in isolates carrying the blaPER-1 gene as compared with isolates without this extended-spectrum β-lactamase gene (p <0.005 and p <0.001, respectively). Further examination by RT-PCR showed a positive correlation between the level of expression of the blaPER-1 gene and the level of biofilm formation (r 0.89, p <0.0001) and cell adhesiveness (r 0.74, p <0.006). Overall, the study demonstrated a high capacity of clinical isolates of MDR A. baumannii to form biofilm and to adhere to respiratory epithelial cells. This feature, combined with multidrug resistance, might contribute to the survival of these organisms and their dissemination in the hospital environment.</abstract><cop>Oxford, UK</cop><pub>Elsevier Ltd</pub><pmid>18005176</pmid><doi>10.1111/j.1469-0691.2007.01842.x</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Acinetobacter baumannii Acinetobacter baumannii - drug effects Acinetobacter baumannii - genetics Acinetobacter baumannii - physiology Acinetobacter Infections - microbiology adherence Anti-Bacterial Agents - pharmacology Bacterial Adhesion - physiology Bacteriology beta-Lactamases - genetics biofilm formation on epithelial cells Biofilms - drug effects Biofilms - growth & development Biological and medical sciences Cell Line Drug Resistance, Multiple, Bacterial Edetic Acid - pharmacology Epithelial Cells - microbiology Fundamental and applied biological sciences. Psychology Humans Microbiology multidrug resistance Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains PER-1 β-lactamase Respiratory Mucosa - microbiology |
| title | Capacity of multidrug-resistant clinical isolates of Acinetobacter baumannii to form biofilm and adhere to epithelial cell surfaces |
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