Matrix-assisted peptide synthesis on nanoparticles

We report a new method for multistep peptide synthesis on polymeric nanoparticles of differing sizes. Polymeric nanoparticles were functionalized via their temporary embedment into a magnetic inorganic matrix that allows multistep peptide synthesis. The matrix is removed at the end of the process for obtaining nanoparticles functionalized with peptides. The matrix‐assisted synthesis on nanoparticles was proved by generating various biologically relevant peptides. Copyright © 2014 European Peptide Society and John Wiley & Sons, Ltd. Biocompatible nanoparticles (NPs) are embedded in a magnetic matrix that allows multistep peptide synthesis on the NPs as well as introduction of fluorescent probes. The matrix is discarded at the end of the process, and the NPs are recovered intact with the synthetic peptide and the probe on their surface. The method can be applied to the synthesis of cell‐sensing nanoparticles.