Surface display of Clonorchis sinensis enolase on Bacillus subtilis spores potentializes an oral vaccine candidate

Highlights • The shuttle genetic engineering system facilitated the development of oral vaccine with Bacillus subtilis stably overexpressing target protein. • Cs enolase derived vaccines administrated in different immunization routes could induce a mixed IgG1/IgG2a immune response. • Killed spores e...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Vaccine 2014-03, Vol.32 (12), p.1338-1345
Hauptverfasser:	Wang, Xiaoyun, Chen, Wenjun, Tian, Yanli, Mao, Qiang, Lv, Xiaoli, Shang, Mei, Li, Xuerong, Yu, Xinbing, Huang, Yan
Format:	Artikel
Sprache:	eng
Schlagworte:	Administration, Oral Allergy and Immunology Animals Antibodies, Helminth Applied microbiology Bacillus subtilis Bacillus subtilis - immunology Bacillus subtilis - metabolism Bacteriology Biological and medical sciences Clonorchiasis - prevention & control Clonorchis sinensis Clonorchis sinensis - enzymology Enolase Epitopes, B-Lymphocyte - immunology Excretory–secretory products Fundamental and applied biological sciences. Psychology Genetic Engineering Genetic Vectors Immunity, Cellular Microbiology Miscellaneous Oral vaccine Phosphopyruvate Hydratase - biosynthesis Phosphopyruvate Hydratase - immunology Rats, Sprague-Dawley Recombinant Fusion Proteins - biosynthesis Recombinant Fusion Proteins - immunology Shuttle vector Spores, Bacterial - immunology Spores, Bacterial - metabolism Vaccines - immunology Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Highlights • The shuttle genetic engineering system facilitated the development of oral vaccine with Bacillus subtilis stably overexpressing target protein. • Cs enolase derived vaccines administrated in different immunization routes could induce a mixed IgG1/IgG2a immune response. • Killed spores expressing Cs enolase could trigger reactive Th1/Th2 immune response and exhibited protective efficacy against C. sinensis infection.
ISSN:	0264-410X 1873-2518
DOI:	10.1016/j.vaccine.2014.01.039