Gene Cloning and Alternative Splicing of CCoAOMT Subfamily Genes from Eucalyptus camaldulensis

In this study, the homology-based RT-PCR method and the rapid amplification of cDNA ends (RACE) method were used to clone full-length cDNAs of two CCoAOMT subfamily genes in Eucalyptus camaldulensis. The full-length cDNA of CCoAOMT1 gene is 1 020 bp and contains 738 bp ORF, 106 bp 5' -UTR and 1...

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Veröffentlicht in:Linye kexue (1979) 2014-01, Vol.50 (5), p.62-69
Hauptverfasser: Gu, Zhenjun, Zhang, Huaiyun, Zhang, Dangquan, Xie, Yaojian, He, Hanjie, Chen, Lili, Peng, Kuan, Liu, Guo, Yang, Dan
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Sprache:chi
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Zusammenfassung:In this study, the homology-based RT-PCR method and the rapid amplification of cDNA ends (RACE) method were used to clone full-length cDNAs of two CCoAOMT subfamily genes in Eucalyptus camaldulensis. The full-length cDNA of CCoAOMT1 gene is 1 020 bp and contains 738 bp ORF, 106 bp 5' -UTR and 176 bp 3' -UTR, and the full-length cDNA of CCoAOMT2 gene is 1 047 bp and contains 741 bp ORF, 125 bp 5' -UTR and 158 bp 3' -UTR. The alignment result showed that CCoAOMT1 and CCoAOMT2 genomic DNA both contain five exons. Their main difference lay in that there was a alternative 5' splice site at the CCoAOMT1's first intron, which could result in formation of two mRNA with different lengths. One mRNA contains 738 bp ORF, and the other has a 42 bp deletion, however this splicing doesn't change the reading frame sequence of posterior amino acids. The alternative splicing of CCoAOMT1 gene expresses from May to September, at the most vigorous growing stages of Eucalyptus stem, suggesting that this alternative splicing may in
ISSN:1001-7488
DOI:10.11707/j.1001-7488.20140508