ISOLATION AND PARTIAL CHARACTERIZATION OF LIPASE FROM BACILLUS PSEUDOFIRMUS FROM ALKALINE LONAR LAKE

Alkaline Lonar Lake is a unique ecosystem situated in the Buldhana District of the Maharashtra State, India, and harbors various haloalkaliphilic bacterial bacillus species which produces biotechnologically important thermo-haloalkaliphilic enzymes. In this paper, the bacterial strains BS1(1), CS4(1) and DW1(1) were isolated and screened for production and partial characterizations of lipase. The bacterial Isolates were characterized and identified as Bacillus pseudofirmus BS1(1), B. seudofirmus CS4(1) and B. pseudofirmus DW1(1). Alkaline lipase production was maximum at pH 9 and activity was 1.54 unit/mL to 1.66 unit/mL. Lipase from these bacterial strains was is active at higher temperature and pH and finds potential applications in food, pharmaceutical and detergent industries.