Covalently trapping MutS on DNA to study DNA mismatch recognition and signaling

The DNA repair protein MutS forms clamp-like structures on DNA that search for and recognize base mismatches leading to ATP-transformed signaling clamps. In this study, the mobile MutS clamps were trapped on DNA in a functional state using single-cysteine variants of MutS and thiol-modified homodupl...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Molecular bioSystems 2012-07, Vol.8 (7), p.1861-1864
Hauptverfasser:	Heinze, Roger J, Sekerina, Svetlana, Winkler, Ines, Biertümpfel, Christian, Oretskaya, Tatiana S, Kubareva, Elena, Friedhoff, Peter
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Pair Mismatch DNA Mismatch Repair DNA, Bacterial - chemistry DNA, Bacterial - metabolism DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism MutS DNA Mismatch-Binding Protein - chemistry MutS DNA Mismatch-Binding Protein - metabolism Nucleic Acid Heteroduplexes - genetics Nucleic Acid Heteroduplexes - metabolism Signal Transduction
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The DNA repair protein MutS forms clamp-like structures on DNA that search for and recognize base mismatches leading to ATP-transformed signaling clamps. In this study, the mobile MutS clamps were trapped on DNA in a functional state using single-cysteine variants of MutS and thiol-modified homoduplex or heteroduplex DNA. This approach allows stabilization of various transient MutS-DNA complexes and will enable their structural and functional analysis.
ISSN:	1742-206X 1742-2051
DOI:	10.1039/c2mb25086a