Assembling the Protein Architecture of the Budding Yeast Kinetochore-Microtubule Attachment using FRET

The kinetochore is a multiprotein machine that couples chromosome movement to microtubule (MT) polymerization and depolymerization. It uses numerous copies of at least three MT-binding proteins to generate bidirectional movement. The nanoscale organization of these proteins within the kinetochore plays an important role in shaping the mechanisms that drive persistent, bidirectional movement of the kinetochore. We used fluorescence resonance energy transfer (FRET) between genetically encoded fluorescent proteins fused to kinetochore subunits to reconstruct the nanoscale organization of the budding yeast kinetochore. We performed >60 FRET and high-resolution colocalization measurements involving the essential MT-binding kinetochore components: Ndc80, Dam1, Spc105, and Stu2. These measurements reveal that neighboring Ndc80 complexes within the kinetochore are narrowly distributed along the length of the MT. Dam1 complex molecules are concentrated near the MT-binding domains of Ndc80. Stu2 localizes in high abundance within a narrowly defined territory within the kinetochore centered ∼20 nm on the centromeric side of the Dam1 complex. Our data show that the MT attachment site of the budding yeast kinetochore is well organized. Ndc80, Dam1, and Stu2 are all narrowly distributed about their average positions along the kinetochore-MT axis. The relative organization of these components, their narrow distributions, and their known MT-binding properties together elucidate how their combined actions generate persistent, bidirectional kinetochore movement coupled to MT polymerization and depolymerization. •Ndc80 complexes are narrowly distributed within the budding yeast kinetochore•Dam1 complex molecules concentrate near microtubule-binding domains of Ndc80•Stu2 molecules localize dynamically proximal to the centromeric end of the Ndc80•This organization suggests a cohesive model of bidirectional kinetochore movement Aravamudhan et al. define the nanoscale distributions of kinetochore proteins using FRET and reconstruct the architecture of the kinetochore-microtubule attachment. They propose a cohesive mechanism for generating kinetochore movement coupled to microtubule polymerization and depolymerization.