Construction and evaluation of replication-defective recombinant optimized triosephosphate isomerase adenoviral vaccination in Schistosoma japonicum challenged mice

Highlights • We constructed the rAdV-SjTPI.opt adenoviral vector based vaccine and tested its efficacy against Schistosoma japonicum infection. • Intramuscular rAdV-SjTPI.opt induced Th1 biased immune responses in the host, while subcutaneous rAdV-SjTPI.opt induced Th2 predominant immune responses. • Oral rAdV-SjTPI.opt induced low levels of immune responses and no significant protection. • Intramuscular rAdV-SjTPI.opt provided a consistent and repeatable higher protective effect in mice. • Associated higher levels of specific Th1, antibody responses and lower level of IL-17A may contribute to the higher protective effect.