A Selective Immunosensor for D-dimer Based on Antibody Immobilized on a Graphene Electrode with Incorporated Lipid Films

The present article describes a miniaturized potentiometric D‐dimer biosensor on graphene nanosheets with incorporated lipid films. The graphene electrode was used for the development of a very selective and sensitive immunosensor for the detection of D‐dimer by immobilizing the mouse anti human D‐dimer antibody on stabilized lipid films. The immunosensor responded for the wide range of D‐dimer concentrations with fast response time of ca. 15 s. The presented potentiometric D‐dimer biosensor is easy to construct and exhibits good reproducibility, reusability, selectivity, rapid response times, long shelf life and high sensitivity of ca. 59 mV/decade over the D‐dimer logarithmic concentration range from 10−6 μg/L to 10−3 μg/L.