Poly-ε-Lysine Modified Nanocarbon Film Electrodes for LPS Detection

Poly-ε-Lysine Modified Nanocarbon Film Electrodes for LPS Detection

We developed an electrochemical system for detecting lipopolysaccharide (LPS) that uses an ultraflat nanocarbon film electrode modified with poly‐ε‐lysine with a high affinity to LPS. LPS was captured on the modified electrode, and then ferrocene labeled polymyxin B (FcPMB) was captured on the LPS a...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Electroanalysis (New York, N.Y.) N.Y.), 2014-03, Vol.26 (3), p.618-624
Hauptverfasser: Kato, Dai, Oda, Atsumu, Tanaka, Mutsuo, Iijima, Seiichiro, Kamata, Tomoyuki, Todokoro, Masami, Yoshimi, Yasuo, Niwa, Osamu
Format: Artikel
Sprache:eng
Schlagworte:
Affinity
Amplification
Electrochemical detection
Electrodes
Electrolytic analysis
Ferrocenes
Lipopolysaccharide
Low noise
Nanocarbon film electrode
Nanostructure
Poly-ε-lysine
Signal amplification
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:We developed an electrochemical system for detecting lipopolysaccharide (LPS) that uses an ultraflat nanocarbon film electrode modified with poly‐ε‐lysine with a high affinity to LPS. LPS was captured on the modified electrode, and then ferrocene labeled polymyxin B (FcPMB) was captured on the LPS adsorbed electrode via the LPS‐PMB affinity interaction. The adsorbed FcPMB provided an amplified response with Fe2+ ions, and the current response was dependent on the amount of captured LPS (LOD=2.0 ng/mL). This was due to the efficient accumulation of the obtained current for LPS and the very low noise made possible by the ultraflat surface.
ISSN:1040-0397
1521-4109
DOI:10.1002/elan.201300542