Variation in high molecular weight glutenin subunits of Triticum aestivum and T. turgidum ssp. dicoccoides

Two-dimensional electrophoresis revealed biochemical variation for glu-B1 high molecular weight (HMW) glutenin subunit 7 present in Dutch wheat (Triticum aestivum ) varieties. This variation was not recognized by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). In T. turgidum ssp. dicoccoides , HMW glutenin subunits were detected which do not occur in varieties. Some of these subunits show similar relative mobilities during SDS-PAGE as subunits present in varieties and as such unambiguous identification is not possible.