Autofluorescence spectroscopy for NADH and flavoproteins redox state monitoring in the isolated rat heart subjected to ischemia-reperfusion
Highlights • New methodology of NADH and FAD autofluorescence registration has been described. • The changes in NADH and FAD autofluorescence during ischemia were opposite. • Spatial heterogeneity of autofluorescence was found in ischemia-reperfusion. • Several brief episodes of ischemia-reperfusion...
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| Veröffentlicht in: | Photodiagnosis and photodynamic therapy 2014-09, Vol.11 (3), p.400-408 |
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| container_title | Photodiagnosis and photodynamic therapy |
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| creator | Papayan, Garry Petrishchev, Nickolay Galagudza, Michael, MD |
| description | Highlights • New methodology of NADH and FAD autofluorescence registration has been described. • The changes in NADH and FAD autofluorescence during ischemia were opposite. • Spatial heterogeneity of autofluorescence was found in ischemia-reperfusion. • Several brief episodes of ischemia-reperfusion modified autofluorescence changes. • Oscillations of the signal at the beginning of ischemia or reperfusion were found. |
| doi_str_mv | 10.1016/j.pdpdt.2014.05.003 |
| format | Article |
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| ispartof | Photodiagnosis and photodynamic therapy, 2014-09, Vol.11 (3), p.400-408 |
| issn | 1572-1000 1873-1597 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Autofluorescence Flavoproteins - metabolism Heart Hematology, Oncology and Palliative Medicine In Vitro Techniques Internal Medicine Ischemia Male Myocardial Reperfusion Injury - metabolism Myocardium - metabolism NAD - metabolism Nicotinamide adenine dinucleotide Oxidation-Reduction Photodiagnosis Rats Rats, Wistar Spectrometry, Fluorescence - methods Spectroscopy |
| title | Autofluorescence spectroscopy for NADH and flavoproteins redox state monitoring in the isolated rat heart subjected to ischemia-reperfusion |
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