Autofluorescence spectroscopy for NADH and flavoproteins redox state monitoring in the isolated rat heart subjected to ischemia-reperfusion

Highlights • New methodology of NADH and FAD autofluorescence registration has been described. • The changes in NADH and FAD autofluorescence during ischemia were opposite. • Spatial heterogeneity of autofluorescence was found in ischemia-reperfusion. • Several brief episodes of ischemia-reperfusion...

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Veröffentlicht in:	Photodiagnosis and photodynamic therapy 2014-09, Vol.11 (3), p.400-408
Hauptverfasser:	Papayan, Garry, Petrishchev, Nickolay, Galagudza, Michael, MD
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Autofluorescence Flavoproteins - metabolism Heart Hematology, Oncology and Palliative Medicine In Vitro Techniques Internal Medicine Ischemia Male Myocardial Reperfusion Injury - metabolism Myocardium - metabolism NAD - metabolism Nicotinamide adenine dinucleotide Oxidation-Reduction Photodiagnosis Rats Rats, Wistar Spectrometry, Fluorescence - methods Spectroscopy
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Beschreibung
Zusammenfassung:	Highlights • New methodology of NADH and FAD autofluorescence registration has been described. • The changes in NADH and FAD autofluorescence during ischemia were opposite. • Spatial heterogeneity of autofluorescence was found in ischemia-reperfusion. • Several brief episodes of ischemia-reperfusion modified autofluorescence changes. • Oscillations of the signal at the beginning of ischemia or reperfusion were found.
ISSN:	1572-1000 1873-1597
DOI:	10.1016/j.pdpdt.2014.05.003