Mutagenesis directed by phosphotriester analogues of oligonucleotides: A way to site-specific mutagenesis in vivo

A new approach to induce directed mutations in genes of study through simple cotransfection of E. coli cells by the mixture of primer and template was developed. This method is based on the use of synthetic phosphotriester analogues of oligonucleotides as site-specific mutagenic primers. The achieve...

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Veröffentlicht in:	FEBS letters 1988-09, Vol.238 (1), p.109-112
Hauptverfasser:	Petrenko, V.A., Kipriyanov, S.M., Boldyrev, A.N., Pozdnyakov, P.I.
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Biological and medical sciences Coliphages - genetics DNA sequencing DNA, Single-Stranded - genetics DNA, Viral - genetics Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Genetic Engineering - methods Molecular and cellular biology Molecular genetics Molecular Sequence Data Mutagenesis Mutagenesis. Repair Mutation Oligonucleotide Probes Oligonucleotide-directed mutagenesis Phage M13 Phosphorylation single-stranded DNA Templates, Genetic Transfection β-Galactosidase
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Zusammenfassung:	A new approach to induce directed mutations in genes of study through simple cotransfection of E. coli cells by the mixture of primer and template was developed. This method is based on the use of synthetic phosphotriester analogues of oligonucleotides as site-specific mutagenic primers. The achieved yield of mutant clones was 2–3%.
ISSN:	0014-5793 1873-3468
DOI:	10.1016/0014-5793(88)80236-9