The transmembrane topology of the α subunit from the ATPase in Escherichia coli analyzed by PhoA protein fusions

The atpB gene encodes the α subunit of the H +-ATPase of E. coli. The topology of this membrane protein has been analyzed by PhoA fusions. The results support an eight transmembrane segment model that is consistent with the hydropathic profile.

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Bibliographische Detailangaben
Veröffentlicht in:	FEBS letters 1990-01, Vol.260 (1), p.31-34
Hauptverfasser:	Bjørbæk, Christian, Foërsom, Vibeke, Michelsen, Ole
Format:	Artikel
Sprache:	eng
Schlagworte:	adenosinetriphosphatase Amp, ampicillin bla, β-lactamase gene Exact sciences and technology F 0-ATPase F0, membrane part of the ATPase complex F0-ATPase F1, cytoplasmic ATPase part of the H+ -ATPase complex genes Km, kanamycin Mathematical analysis Mathematics PAGE, polyacrylamide gel electrophoresis PhoA protein-fusion PhoA, alkaline phosphatase enzyme Potential theory Sciences and techniques of general use SDS, sodium dodecyl sulfate Subunit α topology X-P, 5-bromo-4-chloro-3-indolylphosphate
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Beschreibung
Zusammenfassung:	The atpB gene encodes the α subunit of the H +-ATPase of E. coli. The topology of this membrane protein has been analyzed by PhoA fusions. The results support an eight transmembrane segment model that is consistent with the hydropathic profile.
ISSN:	0014-5793 1873-3468
DOI:	10.1016/0014-5793(90)80058-Q