Extraction and quantitation of astaxanthin from Phaffia rhodozyma

The rapid, quantitative release of astaxanthin and other carotenoids from the yeast Phaffia rhodozyma is described. Washed cells are ruptured with dimethylsulfoxide (DMSO) and carotenoids extracted into an organic solvent. Extraction and spectrophotometric quantitation of total carotenoids is rapid,...

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Veröffentlicht in:	Biotechnology techniques 1990-01, Vol.4 (2), p.107-112
Hauptverfasser:	Sedmak, J.J, Weerasinghe, D.K, Jolly, S.O
Format:	Artikel
Sprache:	eng
Schlagworte:	astaxanthin Biological and medical sciences Biotechnology carotenoids Deuteromycotina extraction feed supplements Fundamental and applied biological sciences. Psychology high performance liquid chromatography Methods. Procedures. Technologies Others salmon culture Various methods and equipments
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Zusammenfassung:	The rapid, quantitative release of astaxanthin and other carotenoids from the yeast Phaffia rhodozyma is described. Washed cells are ruptured with dimethylsulfoxide (DMSO) and carotenoids extracted into an organic solvent. Extraction and spectrophotometric quantitation of total carotenoids is rapid, reproducible and only small volumes (0.1-2 ml) of culture are required. HPLC analysis in normal phase silica gel column indicates that astaxanthin comprises 65-95% of the total pigmented carotenoids of P. rhodozyma .
ISSN:	0951-208X 1573-6784
DOI:	10.1007/bf00163282