Botulinum C2 toxin treatment increases the G-actin pool in intact chicken cells: A model for the cytopathic action of actin-ADP-ribosylating toxins

Botulinum C2 toxin ADP-ribosylates actin in intact chicken embryo cells in a concentration-dependent manner. This effect correlates with an enhancement in the inhibitory potency of the respective cell lysates on DNAase I activity, indicating an increase in the cellular G-actin content of toxin-treat...

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Veröffentlicht in:	Toxicon (Oxford) 1989, Vol.27 (9), p.989-993
Hauptverfasser:	Aktories, K., Reuner, K.-H., Presek, P., Bärmann, M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Actins - metabolism Adenosine Diphosphate - metabolism Animals Bacteriology Biological and medical sciences Botulinum Toxins - pharmacology Cell Survival - drug effects Cells, Cultured Chick Embryo Deoxyribonuclease I - metabolism Fundamental and applied biological sciences. Psychology Microbiology Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains Ribose - metabolism
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Zusammenfassung:	Botulinum C2 toxin ADP-ribosylates actin in intact chicken embryo cells in a concentration-dependent manner. This effect correlates with an enhancement in the inhibitory potency of the respective cell lysates on DNAase I activity, indicating an increase in the cellular G-actin content of toxin-treated cells. The data support our view, that ADP-ribosylation of cellular actin with subsequent depolymerization of cytoskeleton-associated F-actin to monomeric G-actin is involved in the cytotoxic effects of botulinum C2 toxin. A model of the cytopathic action of actin-ADP-ribosylating toxins is presented.
ISSN:	0041-0101 1879-3150
DOI:	10.1016/0041-0101(89)90149-9